The tumour suppressor effects and regulation of cancer stem cells by macrophage migration inhibitory factor targeted miR-451 in colon cancer

Gene
Afraa MamooriAlfred King-Yin Lam

Abstract

This study aimed to investigate the impact of miR-451 on the biological behaviours of colon cancer cells along with its targets interactions. The levels of miR-451 were tested in colon cancer cell lines (SW480 and SW48). Multiple functional and immunological assays were performed to analyse miR-451 induced growth changes in-vitro and downstream effects on target proteins. Overexpression of miR-451 in colon cancer cells led to reduced cell proliferation, increased apoptosis and decrease accumulation of the cells at the G0/G1 phase of the cell cycle. In addition, a significant increase in the number of the cells was noted in the G2-M phase of cell cycle. Moreover, miR-451 reduced the expression of Oct-4, Sox-2 and Snail indicating its role in stem cell and epithelial-mesenchymal transition (EMT) regulation. An inverse correlation between miR-451 and macrophage migration inhibitory protein (MIF) protein expression occurred in colon cancer cells. Furthermore, restoration the level of miR-451 in colon cancer cells inhibits tumour spheres formation. miR-451 has tumour suppressor effects in vitro, which can inhibit the cancer-related signalling pathways in colon cancer.

Citations

Mar 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eugenio CavalliMaria Sofia Basile
Feb 15, 2021·Current Oncology Reports·Ichiro Abe, Alfred King-Yin Lam
Feb 11, 2021·International Journal of Molecular Sciences·Andrea AngiusMaria Rosaria De Miglio
May 14, 2021·Frontiers in Cell and Developmental Biology·Stefania BrunoGiovanni Camussi
Jun 11, 2021·Molecular Medicine Reports·Chunrong WuDebing Xiang
Sep 30, 2021·Expert Reviews in Molecular Medicine·Sepideh MirzaeiHaroon Khan
Sep 14, 2021·MicroRNA·Saiedeh Razi SoofiyaniTohid Ghasemnejad
Jan 29, 2022·Molecular and Cellular Biochemistry·Bernard NsengimanaShaoping Ji

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