PMID: 2121709Nov 1, 1990Paper

The two-component regulatory system ompR-envZ controls the virulence of Shigella flexneri

Journal of Bacteriology
M L BernardiniP J Sansonetti

Abstract

In Shigella flexneri, the ompB locus (containing the ompR and envZ genes) was found to modulate expression of the vir genes, which are responsible for invasion of epithelial cells. vir gene expression was markedly enhanced under conditions of high osmolarity (300 mosM), similar to that encountered in tissues both extra- and intracellularly. Two ompB mutants were constructed and tested for virulence and for osmotic regulation of vir genes. An envZ::Tn10 mutant remained invasive, although its virulence was significantly decreased as a result of its inability to survive intracellularly. By using a vir::lac operon fusion, this mutation was shown to decrease beta-galactosidase expression both in low- and high-osmolarity conditions but did not affect vir expression in response to changes in osmolarity. A delta ompB deletion mutant was also constructed via allelic exchange with an in vitro-mutagenized ompB locus of Escherichia coli. This mutation severely impaired virulence and abolished expression of the vir::lac fusion in both low- and high-osmolarity conditions. Therefore, a two-component regulatory system modulates virulence according to environmental conditions. In addition, the mutation affecting a spontaneous avirulent variant ...Continue Reading

References

Jan 31, 1979·Molecular & General Genetics : MGG·C VerhoefH Verheij
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M L BernardiniP J Sansonetti
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B AricóR Rappuoli
Nov 1, 1989·Molecular Microbiology·R GrossR Rappuoli
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S ForstM Inouye
Jan 1, 1988·Journal of Bacteriology·J M SlauchT J Silhavy
Jan 1, 1988·Annual Review of Cell Biology·S Forst, M Inouye
Jul 1, 1985·Infection and Immunity·A T MaurelliP J Sansonetti
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A T Maurelli, P J Sansonetti
Apr 1, 1985·Infection and Immunity·E V OaksS B Formal
Sep 1, 1984·Infection and Immunity·A T Maurelli, R Curtiss
Jul 10, 1981·Nucleic Acids Research·D Ish-Horowicz, J F Burke
Mar 1, 1982·Infection and Immunity·P J SansonettiS B Formal
Jan 1, 1984·Infection and Immunity·A T MaurelliR Curtiss
Sep 1, 1980·Journal of Bacteriology·C WandersmanM Schwartz
Apr 1, 1980·Journal of Molecular Biology·M J Casadaban, S N Cohen

❮ Previous
Next ❯

Citations

Feb 1, 1993·Molecular Microbiology·N Ni Bhriain, C J Dorman
Aug 26, 1998·FEMS Microbiology Letters·N DorrellB W Wren
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·S K AmesL J Kenney
May 6, 2014·Journal of Bacteriology·Chelsea D WaddellLaura J Runyen-Janecky
Oct 9, 2018·Frontiers in Cellular and Infection Microbiology·Karolina JaworskaKatarzyna Brzostek
May 20, 2003·Molecular Microbiology·Xiuhong FengLinda J Kenney
Jan 16, 2008·The Journal of Biological Chemistry·Jee Eun RheeLinda J Kenney
May 18, 2011·FEMS Microbiology Letters·Adrianna RaczkowskaKatarzyna Brzostek
Jan 29, 2019·Frontiers in Microbiology·Ritika ChatterjeeDipshikha Chakravortty
Nov 27, 2018·Frontiers in Microbiology·Matthew J Dorman, Charles J Dorman
Jan 1, 1991·Microbiology and Immunology·M Yoshikawa, C Sasakawa
May 15, 2002·FEMS Microbiology Reviews·Roy D Sleator, Colin Hill
Sep 11, 2010·Antonie van Leeuwenhoek·Adrianna RaczkowskaKatarzyna Brzostek
Jul 20, 2010·Interdisciplinary Sciences, Computational Life Sciences·Mahaboobbasha MalickbashaGurusamy Annadurai
Nov 22, 2016·Critical Reviews in Microbiology·Xiaomei HuYanguang Cong
Feb 2, 2018·Applied Microbiology and Biotechnology·Bora ShinWoojun Park
Feb 26, 2020·Antimicrobial Agents and Chemotherapy·Prashant KarkiMehmet A Orman
Jul 17, 2001·Molecular Microbiology·E PérezC Martín
Jun 18, 2014·Infection and Immunity·Hannah E MarmanShelley M Payne
Jan 31, 2018·Frontiers in Microbiology·Zhiwei LinDi Qu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.