The two-component system DcuS-DcuR is involved in virulence and stress tolerance in the poplar canker bacterium Lonsdalea populi

Phytopathology
Zeyang ZhengAi-Ning Li

Abstract

The gram-negative bacterium Lonsdalea populi causes an emerging poplar (Populus × euramericana) canker resulting in severe losses to poplar production in China and Europe. Two-component signal transduction systems play important roles in the regulation of virulence and stress responses in phytopathogenic bacteria, We identified a two-component pair (Lqp2625-Lqp2624) in L. populi, highly homologous to DcuS-DcuR of Escherichia coli. Mutants lacking DcuS or DcuR displayed normal growth while their virulence on poplar twigs was impaired. An inability to produce flagella indicated that DcuS and DcuR are involved in biofilm formation and swimming motility. Moreover, the loss of DcuS or DcuR led to increased sensitivity to oxidative stress and chloramphenicol through downregulation of genes associated with catalases and the multidrug efflux pump, suggesting that the two-component pair contributes to cellular adaptation to oxidative and antibiotic stresses. We identified key domains and putative phosphorylation sites important for virulence and stress responses. Our findings reveal the functions of DcuS-DcuR in virulence and stress responses in L. populi and provide increasing evidence that two-component systems are crucial during the ...Continue Reading

References

Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Oct 6, 2001·Current Opinion in Microbiology·K Poole
Feb 9, 2010·Current Opinion in Microbiology·Yasuhiro GotohRyutaro Utsumi
Sep 28, 2010·Applied and Environmental Microbiology·Jennifer M Colburn-CliffordCaitilyn Allen
Jul 4, 2012·Annual Review of Microbiology·Emily J Capra, Michael T Laub
Nov 30, 2019·Frontiers in Microbiology·Aining Li, Wei He

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