The two membrane segments of leader peptidase partition one by one into the lipid bilayer via a Sec/YidC interface

EMBO Reports
Edith HoubenJoen Luirink

Abstract

We have addressed the mechanism of insertion of both transmembrane segments (TMs) of leader peptidase, a double-spanning protein, into the Escherichia coli inner membrane. Using photo-crosslinking, the first TM (H1) was shown to insert at a Sec-translocon/YidC interface in a fixed orientation. H1 lost its contacts with the Sec-translocon and gained access to lipids near YidC soon after complete exposure outside the ribosome. Following lipid integration, it moved away from the Sec/YidC insertion site. The second TM (H2) inserted and interacted with SecY and YidC in a similar transient fashion. The data are consistent with a linear integration model in which the TMs of polytopic inner membrane proteins move one by one from a Sec/YidC insertion site into the lipid bilayer. We propose that YidC assists the lipid partitioning of single TMs.

References

Aug 19, 2000·Nature·J C SamuelsonR E Dalbey
Oct 29, 2000·Progress in Nucleic Acid Research and Molecular Biology·B Hardesty, G Kramer
Apr 20, 2001·Molecular Microbiology·J W de Gier, J Luirink
Oct 13, 2001·Trends in Microbiology·H Mori, K Ito
Jul 11, 2002·The Journal of Biological Chemistry·Edith N G HoubenJoen Luirink
Dec 9, 2003·Nature·Bert Van den BergTom A Rapoport

❮ Previous
Next ❯

Citations

Apr 2, 2005·Current Opinion in Microbiology·Martin van der LaanArnold J M Driessen
Oct 21, 2010·The Journal of Biological Chemistry·Wouter S P JongJoen Luirink
Feb 1, 2011·Annual Review of Biochemistry·Ross E DalbeyAndreas Kuhn
Nov 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Adnan HasonaL Jeannine Brady
Apr 24, 2013·The Journal of Biological Chemistry·Ilie SachelaruHans-Georg Koch
Oct 29, 2005·Journal of Molecular Biology·Sebastian ReifJoachim Rassow
Dec 29, 2011·Biochimica Et Biophysica Acta·Joen LuirinkJan-Willem de Gier
Jan 19, 2012·FEMS Microbiology Reviews·Ross E Dalbey, Andreas Kuhn
Jun 6, 2009·Journal of Molecular Biology·Stefan KolArnold J M Driessen
Jan 14, 2018·Scientific Reports·Narcis-Adrian PetrimanHans-Georg Koch
Sep 28, 2005·The Journal of Biological Chemistry·Sandra DeitermannHans-Georg Koch
Feb 17, 2006·The Journal of Biological Chemistry·Edwin van BlooisJoen Luirink
Mar 4, 2006·The Journal of Biological Chemistry·David J F du PlessisArnold J M Driessen
May 6, 2008·The Journal of Biological Chemistry·Samuel WagnerJan-Willem de Gier
Jan 27, 2005·The Journal of Biological Chemistry·Edwin van BlooisJoen Luirink
Feb 5, 2008·Nature Reviews. Microbiology·Kun Xie, Ross E Dalbey
Mar 10, 2017·Scientific Reports·Ilie SachelaruHans-Georg Koch
May 24, 2018·FEMS Microbiology Letters·Ruth SteinbergHans-Georg Koch
May 4, 2021·Frontiers in Molecular Biosciences·Julia OswaldHans-Georg Koch
May 15, 2021·Journal of Molecular Biology·Hena SandhuGunnar von Heijne
Mar 10, 2017·EcoSal Plus·Andreas KuhnRoss E Dalbey
Sep 25, 2021·Nature Reviews. Molecular Cell Biology·Ramanujan S Hegde, Robert J Keenan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.