The two soluble forms of the lipopolysaccharide receptor, CD14: characterization and release by normal human monocytes

European Journal of Immunology
J J DurieuxH W Ziegler-Heitbrock

Abstract

CD14, a glycolipid-anchored membrane glycoprotein, acts as a high affinity lipopolysaccharide receptor on leukocytes. We previously reported that the Mono-Mac-6 cell line releases two different soluble forms of CD14 (sCD14) (Labeta et al., Eur. J. Immunol. 1993. 23: 2144). Here we show that the two sCD14, which we now refer to as sCD14 alpha (low M(r)) and sCD14 beta (high M(r)), are also synthesized and released by normal human monocytes and present in normal plasma. Their mechanism of release was examined by using the Mono-Mac-6 cell line, chinese hamster ovary cell (CHO)/CD14+ transfectants and plasma from paroxysmal nocturnal hemoglobinuria (PNH) patients. It was found that: (1) sCD14 beta is released faster than sCD14 alpha and that the release of the latter is a lengthy process. (2) Monensin blocked the biosynthesis of membrane-bound CD14 (mCD14) and sCD14, additionally, a 50-kDa CD14 polypeptide accumulated in the cell lysate, suggesting that the different forms of CD14 may have a common precursor. (3) Monensin also blocked the release of sCD14 alpha from surface-labeled cells, suggesting that conversion of mCD14 to sCD14 alpha involves a mechanism of endocytosis followed by exocytosis. Interestingly, (4) sCD14 alpha and...Continue Reading

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Citations

Sep 17, 1999·Journal of Hepatology·G L SuS C Wang
Dec 22, 1999·Human Immunology·Z G FridlenderM Schlesinger
Nov 1, 1994·Scandinavian Journal of Immunology·H W Ziegler-HeitbrockM O Labeta
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Nov 20, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Alex P ReinerRussell P Tracy
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Mar 20, 2010·World Journal of Gastroenterology : WJG·Gyongyi Szabo, Shashi Bala
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Feb 17, 2015·Journal of Gastroenterology and Hepatology·Mustafa CengizSehri Elbeg
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Feb 13, 2013·Clinical & Developmental Immunology·Magdalena DruszczynskaWieslawa Rudnicka
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Oct 30, 2001·Acta Odontologica Scandinavica·K VidalA Donnet-Hughes

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