PMID: 11341994May 9, 2001Paper

The two-step model for translesion synthesis: then and now

Mutation Research
B A Bridges

Abstract

The formation of base substitution mutations following exposure of bacteria to ultraviolet light and many other mutagens occurs during translesion synthesis opposite a photoproduct or other lesion in the template strand of DNA. This process requires the UmuD(2)' UmuC complex, only formed to a significant extent in SOS-induced cells. The "two-step" model proposed that there were two steps, insertion of a wrong base (misincorporation) and use of the misincorporated base as a primer for further chain extension (bypass). The original evidence suggested that UmuD(2)' UmuC was needed only for the second step and that in its absence other polymerases such as DNA polymerase III could make misincorporations. Now we know that the UmuD(2)' UmuC complex is DNA polymerase V and that it can carry out both steps in vitro and probably does both in vivo in wild-type cells. Even so, DNA polymerase III clearly has an important accessory role in vitro and a possibly essential role in vivo, the precise nature of which is not clear. DNA polymerases II and IV are also up-regulated in SOS-induced cells and their involvement in the broader picture of translesion synthesis is only now beginning to emerge. It is suggested that we need to think of the chr...Continue Reading

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Citations

Apr 7, 2005·DNA Repair·Mark R AlbertellaMark J O'Connor
Nov 13, 2002·Mutation Research·Bryn A Bridges
Jan 2, 2003·DNA Repair·Mark D SuttonGraham C Walker
May 25, 2006·Annual Review of Microbiology·Takehiko Nohmi
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mark D SuttonGraham C Walker
May 9, 2001·Mutation Research·G C Walker
Jun 1, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hironobu Ikehata
Jul 1, 2005·Critical Reviews in Microbiology·Abram Aertsen, Chris W Michiels
Feb 27, 2007·The Journal of Biological Chemistry·William L NeeleyJohn M Essigmann

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