The Two TpsB-Like Proteins in Anabaena sp. Strain PCC 7120 Are Involved in Secretion of Selected Substrates.

Journal of Bacteriology
Giang NgoEnrico Schleiff

Abstract

The outer membrane of Gram-negative bacteria acts as an initial diffusion barrier that shields the cell from the environment. It contains many membrane-embedded proteins required for functionality of this system. These proteins serve as solute and lipid transporters or as machines for membrane insertion or secretion of proteins. The genome of Anabaena sp. strain PCC 7120 codes for two outer membrane transporters termed TpsB1 and TpsB2. They belong to the family of the two-partner secretion system proteins which are characteristic of pathogenic bacteria. Because pathogenicity of Anabaena sp. strain PCC 7120 has not been reported, the function of these two cyanobacterial TpsB proteins was analyzed. TpsB1 is encoded by alr1659, while TpsB2 is encoded by all5116 The latter is part of a genomic region containing 11 genes encoding TpsA-like proteins. However, tpsB2 is transcribed independently of a tpsA gene cluster. Bioinformatics analysis revealed the presence of at least 22 genes in Anabaena sp. strain PCC 7120 putatively coding for substrates of the TpsB system, suggesting a rather global function of the two TpsB proteins. Insertion of a plasmid into each of the two genes resulted in altered outer membrane integrity and antibioti...Continue Reading

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jan 1, 1987·Methods in Enzymology·K B Mullis, F A Faloona
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·B BölterR Wagner
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·S ReumannK Keegstra
Oct 16, 2003·Trends in Biochemical Sciences·Luis Sánchez-PulidoAlfonso Valencia
Mar 12, 2004·The EMBO Journal·Clasien J OomenPiet Gros
Apr 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bernard ClantinVincent Villeret
Jun 25, 2004·Nucleic Acids Research·Pantelis G BagosStavros J Hamodrakas
Mar 9, 2005·The FEBS Journal·Suncana MoslavacEnrico Schleiff
Jul 26, 2005·Infection and Immunity·Steven M Julio, Peggy A Cotter
Oct 18, 2005·BMC Bioinformatics·Oliver Mirus, Enrico Schleiff
Jun 1, 1990·Applied and Environmental Microbiology·M H ZwieteringK van 't Riet
Apr 6, 2006·Environmental Microbiology·María Antonia MolinaManuel Espinosa-Urgel
Sep 6, 2006·Journal of Bacteriology·Elvira Olmedo-VerdAntonia Herrero
Aug 28, 2007·Journal of Bacteriology·Suncana MoslavacEnrico Schleiff
Sep 23, 2008·Journal of Bacteriology·Kerstin NicolaisenEnrico Schleiff
Mar 3, 2009·Journal of Basic Microbiology·Kerstin NicolaisenEnrico Schleiff
Jun 30, 2009·Biological Chemistry·Françoise Jacob-DubuissonNathalie Saint
Aug 26, 2009·Molecular Microbiology·C BaudF Jacob-Dubuisson
Feb 23, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Raimund HaarmannEnrico Schleiff
May 6, 2011·Molecular Microbiology·Anne-Sophie DelattreFrançoise Jacob-Dubuisson
Nov 9, 2011·Environmental Microbiology·Mara StevanovicEnrico Schleiff
Dec 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jan MitschkeAlicia M Muro-Pastor
Mar 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jack C LeoDirk Linke
May 18, 2012·The Journal of Biological Chemistry·Joanna TrippEnrico Schleiff
Mar 12, 2013·Trends in Microbiology·Zachary C RuheChristopher S Hayes
Jun 19, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Mara StevanovicEnrico Schleiff
Sep 3, 2013·Nature·Nicholas NoinajSusan K Buchanan
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta
Nov 16, 2014·Nucleic Acids Research·Scott Federhen
Dec 17, 2014·Life·María José HuertasFrancisco Javier Florencio
Feb 15, 2015·Molecular & Cellular Proteomics : MCP·Qian XiongJin-dong Zhao
Mar 10, 2015·Life·Nils Schuergers, Annegret Wilde
Mar 18, 2015·Biological Chemistry·Yi-Ching HsuehEnrico Schleiff

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