PMID: 13563548Jul 25, 1958Paper

The two-wavelength method of microspectrophotometry. I. A microspectrophotometer and tests on model systems

The Journal of Biophysical and Biochemical Cytology
M L MENDELSOHN

Abstract

In spite of a number of examples of the apparently successful application of the two-wavelength method of microspectrophotometry to biological materials, a demonstration of the effective transition from the theoretical to the practical aspects of the method has never been given. The following tests were carried out on model systems in order to confirm the practical validity of the method: (1) Spherical droplets of Sudan blue or Sudan IV (varying in volume by as much as 400 times) were measured with the surrounding blank field included and were found to have amounts of chromophore which conformed to the estimated droplet volume to within 3 to 6 per cent. (2) Repeated determinations on the same isolated nucleus gave results which did not vary in spite of wide variations in the area of the photometric field. (3) The results of repeated determinations on the same droplet of dye were unaffected by gross changes in the focus of the objective. The microspectrophotometer on which these measurements were made has been briefly described; it was designed specifically for the two-wavelength method. Some of the details of instrumentation have been discussed, in particular, the effects of non-uniform illumination and the contrasting demands ...Continue Reading

References

Dec 1, 1955·Experimental Cell Research·M J MOSES, J H TAYLOR

❮ Previous
Next ❯

Citations

May 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·W B Looney
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R V Kowles, R L Phillips
May 1, 1967·The Journal of Cell Biology·N S Cohn, P van Duijn
Jul 25, 1958·The Journal of Biophysical and Biochemical Cytology·M L MENDELSOHN
Nov 25, 1958·The Journal of Biophysical and Biochemical Cytology·M L MENDELSOHN, B M RICHARDS
Jul 1, 1961·Acta Physiologica Scandinavica·H EMANUELSSON
Jan 1, 1974·Experimental Cell Research·E A Lapan, H J Morowitz
Jun 18, 1999·American Journal of Botany·J Van't Hof
Feb 1, 1969·Journal of Neurochemistry·B Oderfold-Nowak, S Niemierko
Dec 15, 1976·International Journal of Cancer. Journal International Du Cancer·W M Brown, A A Axelrad
Jul 1, 1966·Journal of Scientific Instruments·E L Alpen
Sep 1, 1971·Wilhelm Roux' Archiv Für Entwicklungsmechanik Der Organismen·Pierre Grellet
Sep 29, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Jan 1, 1978·Progress in Histochemistry and Cytochemistry·M FukudaS Fujita

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.