The tyrosine decarboxylation test does not differentiate Enterococcus faecalis from Enterococcus faecium

Systematic and Applied Microbiology
Angela MarcobalRosario Muñoz

Abstract

According to the current edition of the Bergey's Manual of Systematic Bacteriology [11] the tyrosine decarboxylation test allows the differentiation of enterococci. Tyrosine is decarboxylated to the biogenic amine tyramine by E. faecalis and not by E. faecium strains. In the present study we sequenced the16S rDNA of two tyramine-producing strains, BIFI-56 and BIFI-58, presumptively classified as E. faecalis. Their 16S rDNA were identical to the same fragment from the E. faecium type strain. Several E. faecium strains were then checked for their ability to decarboxylate tyrosine and also a putative tyrosine decarboxylase-coding gene was PCR amplified from these strains. All the strains confirmed as E. faecium produced tyramine and possessed a DNA fragment coding for a putative tyrosine decarboxylase. The concordance of the two methods allows us to conclude that the tyrosine decarboxylase test cannot be used in the differentiation of E. faecalis from E. faecium since at least some E. faecium strains are tyramine producers.

Citations

Mar 1, 2012·Critical Reviews in Food Science and Nutrition·Angela MarcobalRosario Muñoz
Mar 18, 2011·International Journal of Food Microbiology·Estefanía Muñoz-AtienzaCarmen Herranz
May 29, 2007·International Journal of Food Microbiology·José María LandeteRosario Muñoz
Jan 27, 2012·International Journal of Food Microbiology·André BarataVirgílio Loureiro
Jul 14, 2016·Probiotics and Antimicrobial Proteins·Halil Dündar
Jun 7, 2005·Journal of Microbiological Methods·Emmanuel Coton, Monika Coton
Jan 10, 2019·Foods·Federica BarbieriGiulia Tabanelli

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