PMID: 9531565May 16, 1998Paper

The tyrosine kinase p56lck mediates activation of swelling-induced chloride channels in lymphocytes

The Journal of Cell Biology
A Lepple-WienhuesF Lang

Abstract

Osmotic cell swelling activates Cl- channels to achieve anion efflux. In this study, we find that both the tyrosine kinase inhibitor herbimycin A and genetic knockout of p56lck, a src-like tyrosine kinase, block regulatory volume decrease (RVD) in a human T cell line. Activation of a swelling-activated chloride current (ICl-swell) by osmotic swelling in whole-cell patch-clamp experiments is blocked by herbimycin A and lavendustin. Osmotic activation of ICl-swell is defective in p56lck-deficient cells. Retransfection of p56lck restores osmotic current activation. Furthermore, tyrosine kinase activity is sufficient for activation of ICl-swell. Addition of purified p56lck to excised patches activates an outwardly rectifying chloride channel with 31 pS unitary conductance. Purified p56lck washed into the cytoplasm activates ICl-swell in native and p56lck-deficient cells even when hypotonic intracellular solutions lead to cell shrinkage. When whole-cell currents are activated either by swelling or by p56lck, slow single-channel gating events can be observed revealing a unitary conductance of 25-28 pS. In accordance with our patch-clamp data, osmotic swelling increases activity of immunoprecipitated p56lck. We conclude that osmotic s...Continue Reading

References

Apr 1, 1992·The Journal of Physiology·P Doroshenko, E Neher
Apr 1, 1992·The Journal of Membrane Biology·S S Garber
Apr 1, 1991·Neuron·I NishimotoP Gardner
Nov 1, 1989·Pflügers Archiv : European journal of physiology·K KunzelmannR Greger
Nov 1, 1994·The American Journal of Physiology·P S JacksonK Strange
Jun 1, 1993·The Journal of General Physiology·R S LewisM D Cahalan
Oct 1, 1995·The Journal of Membrane Biology·I LevitanS S Garber
Jan 1, 1996·The American Journal of Physiology·P S JacksonK Strange
Sep 1, 1995·The Journal of General Physiology·P E Ross, M D Cahalan
Nov 15, 1996·The Biochemical Journal·F SchliessD Häussinger
Dec 1, 1995·Pflügers Archiv : European journal of physiology·S Sorota
Feb 11, 1998·Physiological Reviews·F LangD Häussinger

❮ Previous
Next ❯

Citations

Nov 30, 2005·Cellular and Molecular Life Sciences : CMLS·M SuzukiT Iwamoto
Dec 20, 2005·The Journal of Membrane Biology·F LangS M Huber
May 11, 2006·The Journal of Membrane Biology·Y OkadaN Takahashi
Sep 28, 2007·Pflügers Archiv : European journal of physiology·E Vázquez-JuárezH Pasantes-Morales
Oct 27, 2009·Apoptosis : an International Journal on Programmed Cell Death·Geneviève Dupéré-MinierJacques Bernier
Mar 27, 2004·European Journal of Pharmacology·Baohong JiangChiyoko Inagaki
Jan 4, 2001·Neurochemistry International·S Morales-MuliaH Pasantes-Morales
Nov 27, 2001·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Alexander A. Mongin, Sergei N. Orlov
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Y Okada, E Maeno
Jun 16, 2012·Nature Reviews. Immunology·Stefan FeskeMurali Prakriya
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·T VoetsB Nilius
Jul 13, 2006·The Journal of Biological Chemistry·John C EdwardsPaul H Schlesinger
Nov 14, 2008·The Journal of Biological Chemistry·Mirkka KoivusaloSergio Grinstein
Jun 1, 2006·Acta Physiologica·H Pasantes-MoralesG Ramos-Mandujano
Oct 24, 2008·Acta Physiologica·I H LambertS F Pedersen
Sep 30, 2010·Acta Physiologica·E K Hoffmann, S F Pedersen
Feb 5, 2002·Annual Review of Physiology·Xinhua Li, Steven A Weinman
Jan 25, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Nadezhda N BarvitenkoRoy E Weber
Nov 6, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Diego VarelaAndrés Stutzin
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Else Kay Hoffmann
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·I SzabòF Lang
Jul 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·E MaenoY Okada
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·M R ShenJ C Ellory
Jan 28, 2010·FEBS Letters·Ildikò SzabòErich Gulbins
Dec 2, 2015·Pflügers Archiv : European journal of physiology·Alexander A Mongin
Jan 8, 2016·Pflügers Archiv : European journal of physiology·Stine F PedersenBernd Nilius
May 19, 2004·FEBS Letters·Sebastian F B TomassenBen C Tilly
Oct 12, 1999·The Journal of Physiology·Y Okada
Jan 28, 2009·The Journal of Physiology·Yasunobu OkadaTomohiro Numata
Oct 13, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Caterina FaggioFrancesca Trischitta
Feb 24, 2007·Archives of Biochemistry and Biophysics·Florian LangErich Gulbins

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.