The tyrosine phosphatase, SHP-1, is involved in bronchial mucin production during oxidative stress

Biochemical and Biophysical Research Communications
Min Kyoung JangYou Sook Cho

Abstract

Mucus hypersecretion is a clinically important manifestation of chronic inflammatory airway diseases, such as asthma and Chronic obstructive pulmonary disease (COPD). Mucin production in airway epithelia is increased under conditions of oxidative stress. Src homology 2 domain-containing protein tyrosine phosphatase (SHP)-1 suppression is related to the development of airway inflammation and increased ROS levels. In this study, we investigated the role of SHP-1 in mucin secretion triggered by oxidative stress. Human lung mucoepidermoid H292 carcinoma cells were transfected with specific siRNA to eliminate SHP-1 gene expression. Cultured cells were treated with hydrogen peroxide (H(2)O(2)), and Mucin 5AC(MUC5AC) gene expression and mucin production were determined. Activation of p38 mitogen activated protein kinase (MAPK) in association with MUC5AC production was evaluated. N-acetylcysteine (NAC) was employed to determine whether antioxidants could block MUC5AC production. To establish the precise role of p38, mucin expression was observed after pre-treatment of SHP-1-depleted H292 cells with the p38 chemical blocker. We investigated the in vivo effects of oxidative stress on airway mucus production in SHP-1-deficient heterozygou...Continue Reading

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Citations

Jul 4, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sidra M HoffmanYvonne M W Janssen-Heininger
Jul 19, 2015·Cytokine·Sarah M McCormick, Nicola M Heller
Mar 1, 2012·Therapeutic Advances in Respiratory Disease·Anna M Sadowska
Mar 29, 2018·Respiratory Research·Hiroyuki AizawaMasakazu Ichinose
Jan 5, 2016·Nature Communications·Luciana BatistaFatima Mechta-Grigoriou
May 31, 2018·American Journal of Respiratory Cell and Molecular Biology·Yael Aschner, Gregory P Downey
Feb 11, 2011·Molecular and Cellular Biochemistry·Hongmei YuJuliy M Perelman
Nov 3, 2017·American Journal of Respiratory Cell and Molecular Biology·Tara V SacoNarasaiah Kolliputi

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