The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses

Genes
Maria Elisa SabatiniAnca Macovei

Abstract

Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is involved in DNA repair pathways as it mends the topoisomerase I-DNA covalent complexes. In plants, a small Tdp1 gene family, composed by Tdp1α and Tdp1β genes, was identified, but the roles of these genes in abiotic stress responses are not fully understood. To investigate their specific stress response patterns, the present study made use of bioinformatic and molecular tools to look into the Tdp1β gene function, so far described only in the plant kingdom, and compare it with Tdp1α gene coding for the canonical, highly conserved α isoform. The expression profiles of Tdp1α and Tdp1β genes were examined under abiotic stress conditions (cold, heat, high osmolarity, salt, and UV-B) in two model species, Arabidopsis thaliana and Medicagotruncatula. The two isoforms of topoisomerase I (TOP1α and TOP1β) were also taken into consideration in view of their known roles in DNA metabolism and cell proliferation. Data relative to gene expression in Arabidopsis were retrieved from the AtGenExpress microarray dataset, while quantitative Real-Time PCR was carried out to evaluate the stress response in M.truncatula cell cultures. These analyses revealed that Tdp1β gene expression was enhanced during the...Continue Reading

References

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Datasets Mentioned

BETA
FJ858738
ME00325
ME00338
ME00327
ME00328
ME00329

Methods Mentioned

BETA
RNA-Seq
transgenics
targeted modifications

Software Mentioned

Plaza
Rotor
ExPaSy Clustal W2
MeV ( Multiple Experiment Viewer )
REST2009
Gene 6000 Series

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