The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple-negative breast cancer cells.

FEBS Letters
Bruno Pereira De CarvalhoChia-Hsin Chan

Abstract

The ubiquitin ligase RNF8 is known to induce epithelial-to-mesenchymal (EMT) transition and metastasis in triple-negative breast cancer (TNBC). Besides EMT, Rho GTPases have been shown as key regulators in metastasis. In this study, we investigated the role of RNF8 in regulating Rho GTPases and cell motility. We find that RNF8 knockdown in TNBC cells attenuates the protein and mRNA levels of Ras homolog family member A (RHOA) and cell division cycle 42 (CDC42). We show that the formation of filopodia, focal adhesions, and the association of focal adhesions to stress fibers is impaired upon RNF8 knockdown. Cell migration is significantly inhibited by RNF8 knockdown. Our study suggests a potential novel role for RNF8 in mediating cell migration in TNBC through regulation of the Rho GTPases RHOA and CDC42.

References

Mar 30, 2001·The Journal of Biological Chemistry·S Tu, R A Cerione
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Feb 14, 2006·Methods in Enzymology·Hong-Rui WangJeffrey L Wrana
Nov 17, 2007·Science·Nadine K KolasDaniel Durocher
May 10, 2008·Nature Reviews. Molecular Cell Biology·Pieta K Mattila, Pekka Lappalainen
Mar 31, 2009·Biochimica Et Biophysica Acta·R KarlssonCord Brakebusch
Aug 24, 2010·Nature Reviews. Molecular Cell Biology·J Thomas ParsonsMartin A Schwartz
Mar 13, 2012·Nature Cell Biology·Wen-Hao YangMuh-Hwa Yang
Apr 10, 2012·Seminars in Cancer Biology·Daniela SpanoMassimo Zollo
May 11, 2012·Trends in Cell Biology·Ming LiuYi Zheng
Nov 28, 2012·Biochemical Society Transactions·Alan Hall
Jul 23, 2013·Biochimica Et Biophysica Acta·Jianxin WeiJing Zhao
Aug 13, 2013·International Journal of Medical Sciences·Yoshiyuki SasakiKazuhiro Yoshida
Jul 19, 2014·Small GTPases·Jose L OrgazVictoria Sanz-Moreno
Sep 14, 2015·Current Opinion in Cell Biology·Anne J Ridley
Nov 1, 2015·Experimental Cell Research·Keith Burridge, Christophe Guilluy
Oct 19, 2016·Nature Reviews. Clinical Oncology·Giampaolo BianchiniLuca Gianni
Jun 5, 2016·Journal of Experimental & Clinical Cancer Research : CR·Jingyu KuangGenze Shao
Dec 20, 2016·PloS One·Marie-Pier GirouardAlyson E Fournier
Jan 17, 2017·Molecular & Cellular Oncology·Hong-Jen LeeChia-Hsin Chan
Feb 12, 2017·Cell·Arthur W LambertRobert A Weinberg
Feb 22, 2017·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Shengli WangYue Zhao
May 17, 2017·The Journal of Experimental Medicine·Yan XiaZhimin Lu
Jul 1, 2017·Cell Death & Disease·Arun MuraliKrishnaraj Rajalingam
Aug 3, 2017·The Journal of Cell Biology·Guillaume JacquemetJohanna Ivaska
Mar 10, 2018·Frontiers in Oncology·Christiana NeophytouPanagiotis Papageorgis
Mar 10, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jianxin WeiJing Zhao
Mar 30, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xing-Hua XiaoLi-Xia Xiong
Jun 3, 2018·Cancer Research·María Del Mar Maldonado, Suranganie Dharmawardhane
Dec 14, 2018·International Journal of Molecular Sciences·Pontus Aspenström
Aug 21, 2019·Oncogene·Julius H Svensmark, Cord Brakebusch
Jan 12, 2020·Experimental Cell Research·Xiaoying GuanZuoyi Jiao
Jan 30, 2020·Biomolecules·Paula Tapial MartínezDaniel Lietha

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Methods Mentioned

BETA
GTPases
GTPase
ubiquitination
transfection
PCR

Software Mentioned

FiloQuant
image j
ImageJ
GraphPad

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