The ubiquitin-like protein MNSFbeta regulates ERK-MAPK cascade.

The Journal of Biological Chemistry
Morihiko Nakamura, Seiji Yamaguchi

Abstract

MNSFbeta is a ubiquitously expressed member of the ubiquitin-like family that has been implicated in various biological functions. Previous studies have demonstrated that MNSFbeta covalently binds to intracellular proapoptotic protein Bcl-G in mitogen-activated murine T cells. In this study, we further investigated the intracellular mechanism of action of MNSFbeta in macrophage cell line, Raw 264.7 cells. We present evidence that MNSFbeta.Bcl-G complex associates with ERKs in non-stimulated Raw 264.7. We found that MNSFbeta.Bcl-G directly bound to ERKs and inhibited ERK activation by MEK1. In Raw 264.7 cells treated with MNSFbeta small interfering RNA (siRNA) lipopolysaccharide (LPS)-induced ERK1/2 activation was enhanced and LPS-induced JNK and p38 activation was unaffected. SiRNA-mediated knockdown of MNSFbeta increased tumor necrosis factor alpha (TNFalpha) expression at mRNA and protein levels in LPS-stimulated Raw 264.7 cells. Finally, we found that transfection with MNSFbeta expression construct resulted in a significant inhibition of LPS-induced ERK activation and TNFalpha production. Co-transfection experiments with MNSFbeta and Bcl-G greatly enhanced this inhibition. Collectively, these findings indicate that MNSFbeta ...Continue Reading

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Citations

Mar 13, 2012·Annual Review of Biochemistry·Annemarthe G van der Veen, Hidde L Ploegh
Sep 21, 2010·Biochemical and Biophysical Research Communications·Morihiko Nakamura, Natsuko Watanabe
Jun 19, 2013·Neuromolecular Medicine·Yang-ja Lee, John M Hallenbeck
Sep 3, 2014·Immunological Investigations·Morihiko NakamuraJun Watanabe
Mar 26, 2009·Molecular Ecology·Christian R VoolstraMónica Medina
Jun 16, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Na LuoYuzhang Wu
Jul 21, 2015·Biochemical and Biophysical Research Communications·Morihiko NakamuraKaori Notsu
May 15, 2007·Circulation Research·Joerg HerrmannAmir Lerman
Jan 1, 2019·Journal of Biochemical and Molecular Toxicology·Morihiko Nakamura, Natsuko Watanabe
Jan 26, 2012·Molecular and Cellular Biochemistry·Morihiko NakamuraNatsuko Watanabe
Jun 20, 2013·Expert Review of Proteomics·Limin ChenIan McGilvray
Sep 2, 2016·Molecular and Cellular Biochemistry·Kaori NotsuMorihiko Nakamura
Feb 3, 2019·Molecular and Cellular Biochemistry·Morihiko NakamuraMai Nakagawa
Nov 14, 2021·Molecular Biology Reports·Morihiko NakamuraMegumi Kono

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