PMID: 9435231Jan 22, 1998Paper

The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme

Proceedings of the National Academy of Sciences of the United States of America
S E SchwarzS Jentsch

Abstract

The ubiquitin-like protein SMT3 from Saccharomyces cerevisiae and SUMO-1, its mammalian homolog, can be covalently attached to other proteins posttranslationally. Conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and -conjugating (E2) enzymes and proceeds via thioester-linked enzyme-ubiquitin intermediates. Herein we show that UBC9, one of the 13 different E2 enzymes from yeast, is required for SMT3 conjugation in vivo. Moreover, recombinant yeast and mammalian UBC9 enzymes were found to form thioester complexes with SMT3 and SUMO-1, respectively. This suggests that UBC9 functions as an E2 in a SMT3/SUMO-1 conjugation pathway analogous to ubiquitin-conjugating enzymes. The role of yeast UBC9 in cell cycle progression may thus be mediated through its SMT3 conjugation activity.

References

Dec 1, 1994·Molecular and Cellular Biology·K R Loeb, A L Haas
Jul 28, 1995·The Journal of Biological Chemistry·R J DohmenA Varshavsky
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·J D LoebG R Fink
Oct 7, 1994·Cell·A Ciechanover
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·M ScheffnerP M Howley
Apr 1, 1994·Molecular and Cellular Biology·C E DabrowskiP A Schaffer
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·O V KovalenkoE I Golub
Oct 18, 1996·The Journal of Biological Chemistry·G HateboerR Bernards
Dec 6, 1996·The Journal of Biological Chemistry·D A WrightR S Geha
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser
Feb 7, 1997·The Journal of Biological Chemistry·C J KhoE Haber
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·H SaitohM Dasso
May 30, 1997·The Journal of Biological Chemistry·T KamitaniE T Yeh
Jul 14, 1997·The Journal of Cell Biology·D GörlichE Izaurralde
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·K TashiroI M Verma
Oct 27, 1997·The Journal of Biological Chemistry·E S Johnson, G Blobel
Aug 22, 2007·Trends in Cell Biology·P R Johnson, M Hochstrasser

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Citations

May 16, 2002·Journal of Cellular Physiology·Keun Il KimChin Ha Chung
May 17, 2011·Parasitology Research·Roberta Verciano PereiraRenata Guerra-Sá
Jul 20, 1999·Molecular and Cellular Endocrinology·W M BaarendsJ A Grootegoed
May 12, 2000·Gene·E T YehT Kamitani
Jul 8, 2000·Trends in Cell Biology·S Jentsch, G Pyrowolakis
Sep 12, 1998·Molecular Cell·J M DesterroR T Hay
Oct 13, 2000·European Journal of Biochemistry·T NishidaH Yasuda
Nov 23, 2005·The Journal of Experimental Medicine·Honglin ZhouVishva M Dixit
May 8, 1999·Molecular Biology of the Cell·I TanidaE Kominami
Feb 18, 2003·Molecular Biology of the Cell·C D SmithE H Blackburn
Dec 19, 1998·The EMBO Journal·A Ciechanover
May 26, 1998·The EMBO Journal·D LiakopoulosS Jentsch
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R T HayM S Rodriguez
Aug 8, 1998·Genes & Development·F OsakaS Kato
Jun 5, 2003·The Plant Cell·Luisa Maria LoisNam-Hai Chua
Jun 2, 2006·Molecular and Cellular Biology·Alessandra Di BaccoGrace Gill
Jun 22, 2002·Molecular and Cellular Biology·Noora KotajaJorma J Palvimo
May 5, 2005·Molecular and Cellular Biology·Melanie J DobsonJoyce S K Chew
Mar 23, 2004·Annual Review of Immunology·Yun-Cai Liu
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·Maria Almira CorreiaEduardo Mundo-Paredes
Apr 15, 2011·PLoS Pathogens·Gloria ArriagadaStephen P Goff

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