The Ubiquitin-Proteasome System Does Not Regulate the Degradation of Porcine β-Microseminoprotein during Sperm Capacitation.

International Journal of Molecular Sciences
Lucie TumovaPavla Postlerova

Abstract

Sperm capacitation, one of the key events during successful fertilization, is associated with extensive structural and functional sperm remodeling, beginning with the modification of protein composition within the sperm plasma membrane. The ubiquitin-proteasome system (UPS), a multiprotein complex responsible for protein degradation and turnover, participates in capacitation events. Previous studies showed that capacitation-induced shedding of the seminal plasma proteins such as SPINK2, AQN1, and DQH from the sperm surface is regulated by UPS. Alterations in the sperm surface protein composition also relate to the porcine β-microseminoprotein (MSMB/PSP94), seminal plasma protein known as immunoglobulin-binding factor, and motility inhibitor. MSMB was detected in the acrosomal region as well as the flagellum of ejaculated boar spermatozoa, while the signal disappeared from the acrosomal region after in vitro capacitation (IVC). The involvement of UPS in the MSMB degradation during sperm IVC was studied using proteasomal interference and ubiquitin-activating enzyme (E1) inhibiting conditions by image-based flow cytometry and Western blot detection. Our results showed no accumulation of porcine MSMB either under proteasomal inhibi...Continue Reading

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Citations

Apr 4, 2021·International Journal of Molecular Sciences·Manuel Álvarez-Rodríguez, Felipe Martinez-Pastor

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Methods Mentioned

BETA
ubiquitination
flow cytometry
affinity purification
immunoprecipitation
pulldown
affinity
glycosylation
Flow Cytometric
electrophoresis

Software Mentioned

INSPIRE
ZEN
GraphPad Prism
IDEAS

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