PMID: 6469721Jan 1, 1984

The ultrastructural ionic fixation technique localizing acetylcholinesterase activity, reveals simultaneously acetylcholine-like cation in the synaptic vesicles of the motor nerve terminals

Histochemistry
S Tsuji

Abstract

A new cytochemical technique is proposed for side by side localization of acetylcholine and of acetylcholinesterase activity of motor end-plate at ultrastructural level. The technique is based on the simultaneous "ionic fixation" of vesicular acetylcholine and of histochemical copper thiocholine precipitate with phosphomolybdic acid: the molybdic heteropolyanion forms insoluble salts with these two quaternary ammonium cations, providing in situ "acetylcholine phosphomolybdate" and "copper thiocholine phosphomolybdate". Both of them are osmium resistant; the electron dense precipitates allow for a fine localization of acetylcholine and acetylcholinesterase activity at electron microscopic level.

Citations

Jul 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·F F FoldesZ Zarday
Nov 19, 1963·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R COUTEAUX
Apr 1, 1949·Proceedings of the Society for Experimental Biology and Medicine·G B KOELLE, J A FRIEDENWALD

Related Concepts

Structure of Intercostal Muscle
Establishment and Maintenance of Localization
Synaptic Vesicles
ACHE
Sample Fixation
Cations
Acetylcholinesterase Activity
Acetylcholine Sulfate (1: 1)
Cytochemistry
Phosphomolybdic acid

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