The ultrastructural localization of the enzymes related to steroid hormone metabolism in the guinea-pig testis

The Histochemical Journal
G Bara

Abstract

A study of the ultrastructural localization of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), glucose-6-phosphate dehydrogenase (G-6-PD), beta-hydroxybutyrate dehydrogenase (beta-HBD), NADH diaphorase (NADH-D) and NADPH diaphorase (NADPH-D) in the guinea-pig testis is reported. The procedures employed included short immersion or perfusion fixation with aldehydes followed by incubation of small blocks in a tetrazolium salt or a ferricyanide medium. The effects of incubation conditions were investigated, and a reaction medium for the ultracytochemical demonstration of 11 beta-HSD is described. Using suitable controls, evidence for the specificity of the cytochemical reactions is presented. It was found that all the enzymes studied were present in both the Leydig and Sertoli cells of the guinea-pig testis and that the intracellular distribution pattern for each enzyme was independent of the cell type. Using tetrazolium salt techniques, both 3 beta-HSD and 11 beta-HSD activities were localized on or in membranes of smooth endoplasmic reticulum and within the mitochondria. With the ferricyanide techniques, G-6-PD activity was found to be associated mainly with the smooth endopla...Continue Reading

References

Sep 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·A M Moustafa, S B Koritz
Jan 5, 1977·Cell and Tissue Research·C De MartinoP G Natali
Nov 8, 1976·Biochemical and Biophysical Research Communications·M Tencé, M Drosdowsky
Nov 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L BenkoëlP Laffargue
May 1, 1976·Proceedings of the Society for Experimental Biology and Medicine·M J DiminoP P Foa
Apr 19, 1976·Biochemical and Biophysical Research Communications·M J Welsh, J P Wiebe
May 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A M SeligmanR E Plapinger
Sep 1, 1973·The Histochemical Journal·G Bara, W A Anderson
Jun 1, 1973·Histochemie. Histochemistry. Histochimie·J S HankerH G Moore
Oct 1, 1972·Biology of Reproduction·Y S Moon, J I Raeside
Jul 12, 1965·Biochemical and Biophysical Research Communications·G DallnerG E Palade
Apr 1, 1966·The Journal of Cell Biology·H D Fahimi, M J Karnovsky
Jan 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K OgawaH Mayahara
Aug 1, 1965·The Journal of Endocrinology·A H BaillieD M Hart
Jul 1, 1966·The Journal of Endocrinology·A H Baillie, W S Mack
Nov 1, 1966·Endocrinology·D R Koerner
Oct 1, 1968·Endocrinology·T M Jackanicz, D T Armstrong
Jan 12, 1971·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J B BellD Lacy
Dec 1, 1971·The Biochemical Journal·R S Basch, M J Finegold
Aug 1, 1973·The Journal of Cell Biology·M J ChapmanJ A Ontko
Jan 1, 1971·Histochemie. Histochemistry. Histochimie·H M Nissen, H Andersen
Jan 1, 1971·Histochemie. Histochemistry. Histochimie·T K Shnitka, G G Talibi
Jan 1, 1967·Zeitschrift für Zellforschung und mikroskopische Anatomie·C J Flickinger
Jan 1, 1968·Histochemie. Histochemistry. Histochimie·S Kerpel-Fronius, F Hajós
Aug 8, 1967·Biochimica Et Biophysica Acta·H InanoB Tamaoki
Mar 1, 1960·Fertility and Sterility·S J BEHRMAN, G S DUBOFF
Jun 4, 1963·Biochimica Et Biophysica Acta·K W MCKERNS
Jan 1, 1963·Zeitschrift für Zellforschung und mikroskopische Anatomie·J BROKELMANN
Jul 1, 1964·The Journal of Cell Biology·H D FAHIMI, C R AMARASINGHAM
Nov 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T HASHIMOTOM S BURSTONE
Dec 1, 1964·J R Microsc Soc·S BRADBURY, V W STEWARD

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Citations

May 30, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Wilma M FrederiksCornelis J F Van Noorden
Dec 1, 1983·Anatomia, histologia, embryologia·E Schiffmann-WyttenbachM König
Jul 20, 2000·Archives of Biochemistry and Biophysics·T Bernas, J W Dobrucki

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