PMID: 6397471Jan 1, 1984Paper

The ultrastructural organization of prematurely condensed chromosomes

Journal of Cell Science. Supplement
S M GollinP N Rao

Abstract

In an effort to understand the arrangement of the basic 30 nm chromatin fibre within metaphase chromosomes, changes in the organization of prematurely condensed chromosomes (PCC) were examined as a function of progression through the cell cycle. The structural features of PCC observed under the light microscope were compared with those obtained by scanning electron microscopy. PCC with varying levels of condensation were obtained by fusing mitotic HeLa cells with interphase cells synchronized at different times in the cell cycle. PCC from G1 cells are composed of rather tightly packed bundles of tortuous chromatin fibres. The density of fibre packing along the longitudinal axis of G1-phase PCC is lower and less uniform than that of metaphase chromosomes. Early G1 PCC exhibit gyres suggesting a despiralized chromonema. The condensed domains in G1 PCC appear to be organized as supercoiled loops; whereas fibre-sparse domains consist of longitudinal fibres running along the chromosome axis. As cells progressed towards S phase, a greater proportion of highly extended regions containing prominent longitudinal fibres became evident in the PCC. The pulverized appearance of S-phase PCC under the light microscope corresponded to the high...Continue Reading

Citations

Jun 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·R MartinG Wanner
Oct 21, 2010·Molecular Cytogenetics·Joshua B StevensHenry H Heng
Dec 12, 2012·The Journal of Cell Biology·Neil J Ganem, David Pellman
Nov 7, 1987·Journal of Theoretical Biology·M Takahashi
Aug 30, 2016·Cell Biology International·Maria A Kuznetsova, Eugene V Sheval
May 10, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Michelle F Pflumm

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