The UMAG_00031 gene from Ustilago maydis encodes a putative membrane protein involved in pH control and morphogenesis

Archives of Microbiology
Juan Antonio Cervantes-MontelongoJose Ruiz-Herrera

Abstract

We report the characterization of the gene UMAG_00031 from Ustilago maydis, previously identified as upregulated at alkaline pH. This gene is located on chromosome 1 and contains an ORF of 1539 bp that encodes a putative protein of 512 amino acids with an MW of 54.8 kDa. The protein is predicted to contain seven transmembrane domains (TMDs) and a signal peptide suggesting that is located in the cell membrane. Null ΔUMAG_00031 mutants were constructed, and their phenotype was analyzed. The mutant displayed a pleiotropic phenotype suggesting its participation in processes of alkaline pH adaptation independent of the Pal/Rim pathway. Also, it was involved in the dimorphic process induced by fatty acids. These results indicate that the protein encoded by the UMAG_00031 gene possibly functions as a receptor of different signals in the cell membrane of the fungus.

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Methods Mentioned

BETA
electrophoresis
PCR
phosphotransferase

Software Mentioned

COFACTOR
MEGAX
TASSER
TMHMM Server
PROFbval

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