PMID: 2495940Mar 1, 1989Paper

The uncoupling protein dimer can form a disulfide cross-link between the mobile C-terminal SH groups

European Journal of Biochemistry
M Klingenberg, M Appel

Abstract

Isolated uncoupling protein (UCP) can be cross-linked, by various disulfide-forming reagents, to dimers. The best cross-linking is achieved with Cu2+-phenanthroline oxidation. Because cross-linking is independent of UCP concentration and prevented by SDS addition, a disulfide bridge must be formed between the two subunits of the native dimer. Cross-linking is prevented by SH reagent and reversed by SH-reducing reagents. In mitochondria, cross-linking of UCP with disulfide-forming agents is even more efficient than in isolated state. It proves that UCP is a dimer in mitochondria, before isolation. Disulfide-bridge formation does not inhibit GTP-binding to UCP. Cross-linked UCP re-incorporated in proteoliposomes either before or after cross-linking fully retains the H1-transport function. Rapid cross-linking by membrane impermeant reagents indicates a surface localization of the C-terminus in soluble UCP and projection to the outer surface in mitochondria. Intermolecular disulfide-bridge formation in a dimer requires juxtaposition of identical cysteines at the twofold symmetry axis. A rigid juxtaposition of cysteines is unlikely, unless intended for a native disulfide bridge. The absence of such a bridge in UCP suggests that juxt...Continue Reading

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Citations

Sep 18, 2009·Pflügers Archiv : European journal of physiology·Marcelo O Dietrich, Tamas L Horvath
Nov 4, 2008·Journal of Bioenergetics and Biomembranes·Véronique TrézéguetGérard Brandolin
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Sep 26, 2008·IUBMB Life·Hartmut Wohlrab
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Jun 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yang LeePaul G Crichton
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Jul 17, 2015·Bioscience Reports·Tuan HoangMasoud Jelokhani-Niaraki
Dec 17, 2009·Biochemistry·Marina V IvanovaMasoud Jelokhani-Niaraki
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Jan 16, 1999·Biochimica Et Biophysica Acta·M Klingenberg, S G Huang
Aug 10, 2021·The Journal of Physical Chemistry. B·Afshan ArdalanMasoud Jelokhani-Niaraki
Dec 30, 2020·The Journal of Physical Chemistry. B·Afshan ArdalanMasoud Jelokhani-Niaraki
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