PMID: 9657521Jul 10, 1998Paper

The unimportance of being (protein kinase C) epsilon

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
L A WalkerA V Somlyo

Abstract

The purpose of our study was to determine the mechanism through which phorbol esters and smooth muscle myosin phosphatase inhibitors can induce contraction of smooth muscle in the absence of Ca2+. Protein kinase C-epsilon (PKC-epsilon) was previously implicated in this process based largely on its supposed absence in the ferret portal vein, and a correlation was drawn between the presence of this isoform and the ability of smooth muscle to contract independently of Ca2+ and phosphorylation of the 20 kDa regulatory light chains of myosin (MLC20). We demonstrate here, with two antibodies, one to the NH2 terminus and the other to the COOH terminus of PKC-epsilon, that epsilon is present in both ferret portal vein and rabbit portal vein smooth muscle, neither of which exhibits phorbol ester-induced contraction in the absence of Ca2+. However, in the presence of clamped submaximal Ca2+, phorbol es ter increased MLC20 phosphorylation from 17.7+/-1.7% to 46.4+/-3.6% in ferret portal vein smooth muscle and evoked an increase in force. Prolonged (48 h) incubation of ferret portal vein with phorbol esters completely down-regulated PKC-epsilon, as shown by Western blots, and abolished the phorbol ester-evoked contraction at submaximal Ca2...Continue Reading

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Citations

Apr 14, 1999·The Journal of Physiology·A P Somlyo
Apr 2, 2016·Oxidative Medicine and Cellular Longevity·Amy C KellerJane E B Reusch
Oct 23, 2018·Oxidative Medicine and Cellular Longevity·A C KellerJ E B Reusch
Mar 29, 2003·Physiological Reviews·Alan R BurnsDavid C Walker
Jan 17, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Suresh B PatilKhalil N Bitar
Dec 31, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Cassana M LittlerEdward C Dempsey
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Pawel T SzymanskiRaj K Goyal
Jan 15, 1999·Acta Physiologica Scandinavica·A P Somlyo, A V Somlyo
Dec 6, 2008·Journal of Pharmacological Sciences·Toshihide KashiharaTomohisa Ishikawa
Jun 13, 2001·American Journal of Physiology. Cell Physiology·M C GongA P Somlyo
Jul 18, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·K S MurthyG M Makhlouf

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