The unphosphorylated form of the PilR two-component system regulates pilA gene expression in Geobacter sulfurreducens

Environmental Science and Pollution Research International
Alberto Hernández-EligioKaty Juárez

Abstract

In Geobacter sulfurreducens, metal reduction and generation of bioelectricity require the participation of several elements, and among them, the type IV pili has an essential role. The pilus is composed of multiple PilA monomers. Expression of pilA gene depends mainly on the σ54 factor and the response regulator protein PilR. In this work, we characterized the role of the PilS-PilR two-component system in the regulation of the pilA gene expression. Experimental evidence indicates that PilS is autophosphorylated at the His-334 residue, which in turn is transferred to the conserved Asp-53 in PilR. Contrary to other PilS-PilR systems, substitution D53N in PilR resulted in higher activation of the pilA gene. By using a pilA::luxCDABE fusion with different promoter fragments and in vitro DNA-binding assays, we demonstrated the existence of multiple functional PilR binding sites. A regulatory model in which the non-phosphorylated PilR protein directs activation of pilA expression by binding to two sites in the promoter region of this gene is presented.

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Citations

Oct 4, 2020·Journal of Industrial Microbiology & Biotechnology·Morgen M Clark, Gemma Reguera
Oct 21, 2020·Journal of Bacteriology·Katherine J Graham, Lori L Burrows
May 1, 2021·International Journal of Environmental Research and Public Health·Mohammad Faisal UmarNorli Ismail
May 15, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Carmen Gomez-ArrebolaIñigo Lasa

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