The unusual stability of saporin, a candidate for the synthesis of immunotoxins

Biochemical and Biophysical Research Communications
S SantanchéM Brunori

Abstract

Saporin, a monomeric protein extracted from the seeds of Saponaria officinalis, is an enzyme capable of specific depurination of the eukaryotic ribosomes. Because of its toxicity, saporin proved useful for the synthesis of immunotoxins, chimeric conjugates of a toxin and an antibody specifically directed against cancer cells or other targets. In this paper we report a study of the structural properties of saporin in the presence of denaturing agents and/or proteolytic enzymes. We found that saporin is extremely resistant to denaturation by urea or guanidine (up to 4 M), even at relatively high temperature (up to 55 degrees C). Moreover a structural change detected as a reduction of the fluorescence emission of the single Trp residue is reversible and is not paralleled by changes of the far UV CD spectrum, suggesting that even under harsh experimental conditions unfolding is limited. In good agreement with these results, guanidine-treated saporin is not attacked by proteolytic enzymes. The remarkable resistance of saporin to denaturation and proteolysis suggests this protein as an ideal candidate for biotechnological applications.

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Citations

Feb 11, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Flavia AntonucciWolfgang Härtig
Oct 10, 2013·Toxins·Letizia PolitoAndrea Bolognesi
Jun 23, 2015·Biochimica Et Biophysica Acta·Marina SánchezAlessio Ausili
Jan 1, 2008·Protein Expression and Purification·Emine GünhanLeo M Chalupa
Sep 28, 2013·Virulence·Matthew J WalshGuillaume M Hautbergue
Oct 23, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Tracy R Daniels-WellsManuel L Penichet
Nov 30, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrea BolognesiLetizia Polito
Jan 26, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sergey O TcherniukMaxim Y Balakirev
Oct 14, 2017·Toxins·Christina C TamLuisa W Cheng
Feb 14, 2018·Toxins·Francesco GiansantiRodolfo Ippoliti
Jul 15, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Charlene DiepenbroekGuillaume de Lartigue
Jun 24, 2004·Journal of Neuroscience Research·Luc Jasmin, Peter T Ohara
Jan 8, 2005·Korean Journal of Ophthalmology : KJO·Yeong Hoon KimJin Seong Yoo
Sep 30, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alessio LombardiMaria Serena Fabbrini
Sep 25, 2017·Journal of Cellular Physiology·Anne-Chloé DhezRodolfo Ippoliti
Jul 17, 2019·Brain, Behavior, and Immunity·Léa ChaskielJan Pieter Konsman
May 16, 2015·Journal of Cell Science·Maria Daniela Garcia-CastilloLudger Johannes
Sep 8, 2021·Cancer Cell International·Reza MahmoudiMohammad Rahmati

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