The unusual translational initiation codon AUU limits the expression of the infC (initiation factor IF3) gene of Escherichia coli

Molecular & General Genetics : MGG
M Brombach, C L Pon

Abstract

The expression of infC, the structural gene for translational initiation factor IF3, has been studied in different constructs under the control of the lambda PL and tac promoters. The amount of synthesized IF3 has been determined by a quantitative functional test and the levels of IF3-specific mRNA have been estimated. The synthesis of IF3 is strongly enhanced when the unusual AUU initiation codon is changed to AUG by site-directed mutagenesis. Removal of the sequence upstream from the start codon including most of the Shine-Dalgarno sequence, as well as part of a 10 bp region with potential complementarity to an internal region of the 16S rRNA, which is unique to the IF3 mRNA, reduced but did not completely abolish the high expression of infC obtained after introduction of the AUG initiation codon. The level of IF3 mRNA was found to be positively influenced by the presence of the rplT gene in the plasmid downstream from the infC gene. In vivo accumulation of a large excess of IF3, obtained when the infC gene was placed under the control of an incompletely repressed tac promoter, was not accompanied by any noticeable adverse phenotype.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M SpringerH Hennecke
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Dec 21, 1984·Nucleic Acids Research·W KramerH J Fritz
Jan 1, 1980·Methods in Enzymology·A J Smith
Jan 1, 1980·Molecular & General Genetics : MGG·P H O'FarrellM Nakanishi
Jan 24, 1981·Nucleic Acids Research·J MessingP H Seeburg
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jan 1, 1984·Molecular & General Genetics : MGG·H OhsawaC O Gualerzi
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J Brosius, A Holy
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L GoldR Saunders
May 11, 1983·Nucleic Acids Research·T Dörper, E L Winnacker

Citations

May 1, 1991·Molecular & General Genetics : MGG·M FalconiC L Pon
Aug 6, 2003·Journal of Molecular Biology·D PetrelliA La Teana
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A La TeanaC O Gualerzi
Aug 14, 2001·The EMBO Journal·D PetrelliC O Gualerzi
Oct 19, 1999·Biochimica Et Biophysica Acta·Y SagaraH Aramaki
Oct 17, 2002·Protein Science : a Publication of the Protein Society·Jessica J HollenbeckMartha G Oakley
Sep 23, 1997·Protein Science : a Publication of the Protein Society·R M BelagajeW F Prouty
Jan 9, 2004·Protein Expression and Purification·Arun K Mohanty, Michael C Wiener
Jan 25, 2007·Molecular Cell·Attilio FabbrettiC O Gualerzi
May 18, 1999·Journal of Molecular Biology·C SacerdotM Springer
Jun 26, 1990·Biochemistry·C O Gualerzi, C L Pon
Jun 3, 2017·Nucleic Acids Research·He ChengguangC O Gualerzi
Feb 14, 2003·FEMS Microbiology Letters·William J PageBrenda K Leskiw
Dec 1, 1991·FEMS Microbiology Letters·A Romero, P García
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·D PediconiC L Pon
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·D MangrooU L RajBhandary

Related Concepts

DNA Sequence
Sense Codon
Alkalescens-Dispar Group
Peptide Chain Initiation, Translational
Peptide Initiation Factors
Episomes
Early Promoters, Genetic
Poly(A) Tail
15S RNA
Structure-Activity Relationship

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