The UPC2 promoter in Candida albicans contains two cis-acting elements that bind directly to Upc2p, resulting in transcriptional autoregulation.

Eukaryotic Cell
Samantha J HootTheodore C White

Abstract

In Candida albicans, ergosterol biosynthetic genes, including ERG11, which encodes the target of azole antifungal drugs, are regulated by the transcriptional regulator Upc2p. To initially characterize the promoter of the UPC2 gene, 5' rapid amplification of cDNA ends was used to identify two transcriptional initiation sites upstream of the ATG codon. The regions within the UPC2 promoter required for azole regulation of the UPC2 promoter were then identified using nested deletions fused to a luciferase reporter which were tested for azole inducibility in wild-type (WT) and upc2Delta/upc2Delta strains. Two distinct regions important for azole induction were identified: a Upc2p-dependent region (UDR) between bp -450 and -350 upstream of the ATG codon and a Upc2p-independent region (UIR) between bp -350 and -250 upstream of the ATG codon. Within the UDR, loss or mutation of the sterol response element (SRE), so named because of homology to the Saccharomyces cerevisiae Upc2p binding site, resulted in a decrease in both basal and induced expression in the WT strain but did not affect azole inducibility in the upc2Delta/upc2Delta deletion strain. Gel shift analyses using the DNA binding domain of Upc2p confirmed binding of the protein...Continue Reading

References

Oct 12, 2004·Gene·Oliver ReussJoachim Morschhäuser
Sep 28, 2007·Molecular Microbiology·Sadri ZnaidiMartine Raymond
Nov 19, 2008·Antimicrobial Agents and Chemotherapy·Erwin LampingRichard D Cannon

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Citations

Aug 9, 2011·Nature Reviews. Cancer·Marieke van KouwenhoveReuven Agami
Sep 28, 2010·Eukaryotic Cell·John M SynnottGeraldine Butler
Jun 8, 2011·Microbiology and Molecular Biology Reviews : MMBR·Rebecca S ShapiroLeah E Cowen
Oct 23, 2013·Antimicrobial Agents and Chemotherapy·Christina Gallo-EbertJoseph T Nickels
Apr 6, 2017·International Journal of Molecular Sciences·Ifeoluwapo Matthew Joshua, Thomas Höfken
May 29, 2014·Antimicrobial Agents and Chemotherapy·Sarah G WhaleyP David Rogers
Aug 12, 2018·Letters in Applied Microbiology·S-Q WangF Wang
Oct 29, 2013·The Journal of Biological Chemistry·Christina Gallo-EbertJoseph T Nickels

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