Abstract
In this study, with the aid of microarray technology, transmembrane protease serine 4 (TMPRSS4), a novel member of the serine protease family, was found to be upregulated in the majority of lung adenocarcinoma (LUAD) tissues compared to normal lung tissues. Of note, the clinical significance of TMPRSS4 in LUAD has not yet been reported, at least to the best of our knowledge. Through immunohistochemistry assays, we found that TMPRSS4 was overexpressed in LUAD tissues and that the TMPRSS4 expression level was also proportionally associated with the AJCC clinical stage, T stage and pathological grade. Moreover, a high expression of TMPRSS4 was found to be associated with adverse outcomes and was a significant independent factors predicting a poor prognosis. To elucidate the possible mechanisms responsible for the overexpression of TMPRSS4, we examined at microRNAs (miRNAs or miRs), which are small non‑coding RNAs commonly dysregulated in human malignancies and are known to promote carcinogenesis by interacting with other types of RNAs. By means of bioinformatics analysis, a miRNA potentially targeting TMPRSS4 mRNA, namely miR‑125a‑5p, was selected. Dual luciferase reporter gene assays were then performed to verify the interaction....Continue Reading
References
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 27, 2007·Annals of Oncology : Official Journal of the European Society for Medical Oncology·R A Stahel
Jan 3, 2008·Cancer Cell·Sung-Liang YuPan-Chyr Yang
Feb 2, 2010·Carcinogenesis·Semi KimYoung-Kyu Park
Jun 4, 2011·Cancer Gene Therapy·T LiZ-Y Tang
Nov 2, 2011·Respirology : Official Journal of the Asian Pacific Society of Respirology·Humam KadaraIgnacio I Wistuba
Nov 10, 2011·British Journal of Cancer·L LarzabalA Calvo
Feb 11, 2012·International Journal of Oncology·Yojiro HashiguchiMasaki Mori
Jun 14, 2012·International Journal of Oncology·Tri-Hung NguyenSrinivas Shankara
May 11, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mohamed KabboutHumam Kadara
Jul 17, 2013·International Journal of Molecular Sciences·Daye ChengYunhui Li
Aug 27, 2013·Lancet·Martin ReckKlaus F Rabe
Sep 28, 2013·Medical Oncology·Ao HuangMinhua Zheng
Nov 28, 2013·PloS One·Leyre LarzabalAlfonso Calvo
Dec 18, 2013·Cancer Biology & Therapy·Ao HuangMinhua Zheng
Jan 18, 2014·British Journal of Cancer·L LarzabalA Calvo
Feb 6, 2014·Journal of Translational Medicine·Jin-tang XiaWen Li
Apr 22, 2014·Cancer Cell·Taoyong ChenXuetao Cao
Jun 20, 2014·Cancer Investigation·Wang-Yu ZhuYong-Kui Zhang
Sep 10, 2014·British Journal of Cancer·A L de Aberasturi, A Calvo
Sep 23, 2014·Thyroid : Official Journal of the American Thyroid Association·Hongyu GuanYanbing Li
Nov 22, 2014·Cancer Science·Junko HamamotoTomoko Betsuyaku
Dec 17, 2014·Oncotarget·Tsung-Hua HsiehEing-Mei Tsai
May 23, 2015·OncoTargets and Therapy·Ke Yin
Aug 25, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhiguo TongQifan Zhang
Nov 8, 2015·Cancer Letters·Arrate L de AberasturiAlfonso Calvo
Jan 1, 2016·OncoTargets and Therapy·Yi Fu, Fuhua Cao
Jan 15, 2016·Drug Design, Development and Therapy·Xian QinMin Xue
Jan 23, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jie JinLi-ming Zhu
Mar 19, 2016·Oncotarget·Maria VillalbaAlfonso Calvo
Mar 20, 2016·Oncotarget·Manoj K BhasinTowia A Libermann
Oct 28, 2016·OncoTargets and Therapy·Hong ChenXiao-Ning Wei
Mar 16, 2017·Proceedings of the National Academy of Sciences of the United States of America
Jun 21, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lou ZhongZhong Chen