The upstream region of the IPNS gene determines expression during secondary metabolism in Aspergillus nidulans

Gene
E Gómez-Pardo, M A Peñalva

Abstract

We have constructed a translational fusion between the isopenicillin-N-synthetase-encoding gene (IPNS) of Aspergillus nidulans and the lacZ gene of Escherichia coli. Recombinant strains carrying a single copy of the fusion integrated at the IPNS locus produced beta-galactosidase (beta Gal) during secondary metabolism. Integration of the fusion at the argB locus results in a situation in which the only 5'-flanking sequences of the IPNS gene upstream from the chimeric fused gene are those included in the transforming plasmid. Such a strain still expresses beta Gal activity during secondary metabolism, showing that a DNA fragment including sequences of the IPNS gene from nt -2000 to +35 (relative to the translation start codon) still contains sufficient information to drive expression of the fusion gene during secondary metabolism.

References

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Citations

Jan 6, 1995·Molecular & General Genetics : MGG·J M Fernández-Cañón, M A Peñalva
Jul 1, 1993·World Journal of Microbiology & Biotechnology·M A PeñalvaH Martínez-Blanco
Apr 1, 1992·Protein Science : a Publication of the Protein Society·S E BrockerhoffT N Davis
Feb 6, 1998·Journal of Neuroscience Research·P M MathisenJ A Kawczak
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·A A Brakhage

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