The uptake of cardiac glycosides in relation to their actions in isolated cardiac muscle

British Journal of Pharmacology
T Godfraind, M Lesne


1. The uptake of (3)H-digitoxin, (3)H-ouabain and (3)H-dihydro-ouabain by isolated guinea-pig atria has been studied and compared with the inhibition of the sodium pump and with the inotropic effect.2. Analysis of the curve relating the uptake of digitoxin and ouabain at equilibrium to the bath concentration enabled a non-saturable and a saturable binding site to be distinguished.3. The uptake of inactive doses of dihydro-ouabain was only by a non-saturable mechanism.4. The uptake of labelled digitoxin and ouabain was reduced in the presence of another glycoside. The amount of bound glycoside was nearly equivalent to the estimated non-saturable uptake.5. The uptake was reduced at 4 degrees C to the clearance of the non-saturable site.6. ED50 of digitoxin and of ouabain for inhibition of the sodium pump were measured and compared to the ED50 for inotropic effect and to the concentrations producing a half-saturation of the saturable binding site.7. It is concluded that binding to the saturable site may be responsible for the cardiac actions of the glycosides.


Jan 1, 1964·Archives internationales de physiologie et de biochimie·J M Gillis
Jan 1, 1970·Naunyn-Schmiedebergs Archiv Für Pharmakologie·T Godfraind, M Lesne
Jan 1, 1969·The Journal of Pharmacy and Pharmacology·H Lüllmann, P A van Zwieten
Jul 6, 1971·Annals of the New York Academy of Sciences·D S Lukas
Mar 1, 1968·British Journal of Pharmacology and Chemotherapy·K KuschinskyP A van Zwieten
Jun 1, 1967·British Journal of Pharmacology and Chemotherapy·K KuschinskyP A van Zwieten
Apr 1, 1961·The Journal of Physiology·E T DUNHAM, I M GLYNN

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