The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins

Annual Review of Biophysics and Biomolecular Structure
K H Gardner, L E Kay

Abstract

During the past thirty years, deuterium labeling has been used to improve the resolution and sensitivity of protein NMR spectra used in a wide variety of applications. Most recently, the combination of triple resonance experiments and 2H, 13C, 15N labeled samples has been critical to the solution structure determination of several proteins with molecular weights on the order of 30 kDa. Here we review the developments in isotopic labeling strategies, NMR pulse sequences, and structure-determination protocols that have facilitated this advance and hold promise for future NMR-based structural studies of even larger systems. As well, we detail recent progress in the use of solution 2H NMR methods to probe the dynamics of protein sidechains.

References

Jan 1, 1979·Annual Review of Biophysics and Bioengineering·M Das, C F Fox
Aug 1, 1977·Quarterly Reviews of Biophysics·J Seelig
Mar 1, 1992·Journal of Biomolecular NMR·R PachterO Jardetzky
Nov 20, 1991·Journal of Molecular Biology·D S WishartF M Richards
May 1, 1990·Quarterly Reviews of Biophysics·D M LeMaster
May 1, 1990·Quarterly Reviews of Biophysics·J Anglister
Jan 1, 1989·Methods in Enzymology·D M LeMaster
Nov 5, 1988·Journal of Molecular Biology·J JaninC Chothia
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S H SeeholzerH L Crespi
Mar 11, 1966·Science·J J Katz, H L Crespi
Mar 29, 1983·Biochemical and Biophysical Research Communications·G J MartinP Dupuy
Apr 10, 1980·Nature·L W JelinskiD A Torchia
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·J R TolmanJ H Prestegard
Aug 11, 1995·Journal of Molecular Biology·A AszódiW R Taylor
Jun 1, 1995·Journal of Biomolecular NMR·R A VentersC A Fierke
Apr 1, 1995·Current Opinion in Structural Biology·M J Sippl
Feb 1, 1995·Protein Science : a Publication of the Protein Society·M Gochin, H Roder
Aug 1, 1993·Current Opinion in Biotechnology·A G Palmer
Apr 1, 1995·Journal of Biomolecular NMR·E Oldfield
Jun 1, 1995·Journal of Magnetic Resonance. Series B·J KuszewskiG M Clore
Jan 1, 1994·Methods in Enzymology·J KeelerE D Laue
Jan 1, 1994·Methods in Enzymology·G M Clore, A M Gronenborn
Jan 1, 1994·Methods in Enzymology·T L James

❮ Previous
Next ❯

Citations

Nov 19, 2004·Chembiochem : a European Journal of Chemical Biology·Alexander DeitersPeter G Schultz
Jan 29, 2002·Analytical Biochemistry·Nobuhisa ShimbaEi-ichiro Suzuki
Sep 1, 2006·Applied Microbiology and Biotechnology·Oleg Paliy, Thusitha S Gunasekera
Mar 10, 2005·Journal of Biomolecular NMR·Nadine Merkley, Gary S Shaw
Dec 31, 2008·Journal of Biomolecular NMR·Isabel AyalaJérôme Boisbouvier
Mar 17, 2009·Journal of Biomolecular NMR·Nathalie SibilleGuy Lippens
May 27, 2009·Journal of Biomolecular NMR·Chenyun GuoVitali Tugarinov
Aug 20, 2009·Journal of Biomolecular NMR·Lenka SkrisovskaFrédéric H-T Allain
Aug 15, 2009·Journal of Biomolecular NMR·Solmaz SobhanifarVolker Dötsch
Aug 12, 2009·Journal of Biomolecular NMR·David H JonesBernhard H Geierstanger
Dec 4, 2009·Journal of Biomolecular NMR·Chenyun Guo, Vitali Tugarinov
Aug 31, 2010·Journal of Biomolecular NMR·Ming TangChad M Rienstra
Feb 26, 2011·Journal of Biomolecular NMR·Teresa W-M Fan, Andrew N Lane
Sep 29, 2011·Journal of Biomolecular NMR·Teresa MilettiAnthony Mittermaier
Nov 23, 2011·Journal of Biomolecular NMR·Deepak NandMarc Baldus
Jul 26, 2012·Journal of Biomolecular NMR·Joshua M Ward, Nikolai R Skrynnikov
Oct 23, 2012·Journal of Biomolecular NMR·Craig J Markin, Leo Spyracopoulos
Apr 30, 2013·Journal of Biomolecular NMR·Barbara KrähenbühlGerhard Wider
Mar 25, 2010·Journal of Structural and Functional Genomics·William M SchneiderGaetano T Montelione
Jul 1, 2007·Biomolecular NMR Assignments·Sunil PrasannanHelen R Mott
Jul 29, 2009·Biomolecular NMR Assignments·Laura K BaileyHelen R Mott
Mar 24, 2004·Journal of Molecular Biology·César FernándezKurt Wüthrich
Feb 19, 2014·Progress in Nuclear Magnetic Resonance Spectroscopy·Dominique P Frueh
Oct 22, 2003·Current Opinion in Structural Biology·César Fernández, Gerhard Wider
Aug 17, 1999·Current Opinion in Biotechnology·R Bott, R Boelens
Oct 24, 2000·Current Opinion in Structural Biology·N K Goto, L E Kay
Jan 12, 2002·Current Opinion in Structural Biology·A Arora, L K Tamm
Aug 23, 2003·Journal of Magnetic Resonance·Wen-Jin WuJohn L Markley
Aug 14, 2003·Journal of Magnetic Resonance·Leonardus M I KoharudinRolf Boelens
Apr 8, 2010·Quarterly Reviews of Biophysics·Masatsune Kainosho, Peter Güntert
Jun 10, 2011·Analytical Chemistry·Mukundan RagavanChristian Hilty
Jan 11, 2007·Biochemistry·Mark P FosterCarlos D Amero

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
phosphotransferase
x-ray diffraction

Software Mentioned

MOLMOL
MOLSCRIPT

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.