PMID: 107970Mar 23, 1979

The use of 60Co-EDTA as an extracellular marker in frog skin

Biochimica Et Biophysica Acta
K T Ferreira, W M Swensson

Abstract

60Co-EDTA was tested as an extracellular marker in isolated epithelia of frog skin. It was found to be non-toxic for frog skin and 0.1 mM EDTA proved to be enough to saturate any adsorption sites. Comparing with [14C]inulin, 60Co-EDTA marks a slightly greater extracellular space and this volume is constant between 15 min and 2 h. Furthermore it is reproducible and its use simplifies the methodology of the determination of water and electrolyte contents in the cells.

References

Jan 1, 1975·The Journal of Membrane Biology·A D MacknightA Leaf
Jan 1, 1975·The Journal of Membrane Biology·A D MacknightA Leaf
Jan 1, 1973·The Journal of Membrane Biology·J H MorenoM Cereijido
Jun 1, 1971·The Journal of Membrane Biology·A D MacknightM M Civan
Jun 1, 1971·The Journal of Membrane Biology·A D MacknightM M Civan
Dec 1, 1972·The Journal of Membrane Biology·T U BiberP F Curran

Citations

Jun 22, 1983·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H G Ferreira, K T Ferreira

Related Concepts

Kaluril
Salientia
Body Water
Cobalt
Cobalt Radioisotopes
Edetic Acid, Sodium Salt
Mesothelium
Inulin
Ouabain
Potassium

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