The use of 8-aminooctyl sepharose for the separation of some components of the hepatic microsomal electron transfer system

Journal of Biochemistry
Y Imai


Hepatic microsomes from phenobarbital-pretreated rats were treated with sodium cholate in the presence of glycerol and the solubilized proteins were absorbed on a column of 8-aminooctyl derivative of Sepharose 4B. Washing the column stepwise with buffers containing suitable detergents in addition to cholate resulted in the elution of cytochrome P-450, NADH-cytochrome b5 reductase [EC], NADPH-cytochrome c reductase [EC], and cytochrome b5; the former two enzymes were eluted with Emulgen 913 (a polyoxyethylene nonylphenyl ether), and then the latter two were obtained separately by increasing the concentration of added deoxycholate. Thus, cytochrome P-450, NADPH-cytochrome c reductase, and cytochrome b5 were purified to specific contents (or activities) of 9--10 nmoles per mg of protein, 15-16 units per mg of protein, and nearly 35 nmoles per mg of protein, respectively (about 40--50% pure), in 40--50% yields. Practically no mutual contamination of the three enzymes was detected. Benzphetamine N-demethylation activity could be reconstituted on mixing the cytochrome P-450 and NADPH-cytochrome c reductase preparations in the presence of cholate. NADH-cytochrome C reductase activity could be observed at high efficienc...Continue Reading


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Related Concepts

Molecular Sieve Chromatography
Cytochrome P-450 Oxygenase
Cytochrome Reductases
Respiratory Chain
Microsomes, Liver
NADPH-Ferrihemoprotein Reductase
Oxidoreductases, N-Demethylating

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