The use of a cell-cycle phase-marker may decrease the percentage of errors when using FISH in PGD

Cytogenetic and Genome Research
A PujolJ Navarro

Abstract

Fluorescent DNA probes are used to characterise the chromosome constitution of preimplantation embryos. FISH is used to select normal or balanced embryos in carriers of balanced chromosomal rearrangements, for embryo sexing or for aneuploidy screening in women of advanced age, who have had recurrent abortions or IVF failures. In most cases, FISH is performed on interphase blastomeres which are asynchronously dividing cells, that can be in G1, S or G2. However, a correct interpretation of a double FISH signal, which may correspond to a split signal, to a replicated chromosome region or to the presence of an extra chromosome is essential to establish an accurate diagnosis. To determine if the cell stage could influence the interpretation of FISH results, we compared the signal characteristics of one locus-specific probe, two different subtelomere region probes, and a centromere region probe in non-dividing Sertoli cells and in proliferating lymphocytes. Most cells had two signals per chromosome pair (i.e., a situation corresponding to G0 in Sertoli cells and to G1 or to a prereplication stage in lymphocytes). Nevertheless, in proliferating cells the percentage of nuclei with a number of signals different from the expected (two un...Continue Reading

Citations

Sep 8, 2019·Molecular Genetics & Genomic Medicine·Vincent GatinoisJacques Puechberty
Sep 19, 2019·Reproductive Biology and Endocrinology : RB&E·María-José EscribàVanessa Peinado
Jul 31, 2014·Molecular Cytogenetics·Eftychia DimitriadouJoris R Vermeesch

❮ Previous
Next ❯

Related Concepts

Related Feeds