PMID: 6970097Oct 1, 1980Paper

The use of a T lymphocyte colony assay to measure mononuclear phagocyte function

Clinical and Experimental Immunology
M Y GordonE C Gordon-Smith

Abstract

The control of colony formation of normal PHA-stimulated lymphocytes has been investigated using a liquid-on-agar culture system (Lowenberg & De Zeeuw, 1979). Optimal colony formation requires the presence of mononuclear cells which were characterized by their ability to adhere to plastic surfaces. This finding suggests that the assay system can be used to estimate the co-operative function of mononuclear phagocytes as well as to enumerate T lymphocyte colony-forming cells (TL-CFC). Both the capacities of the mononuclear cells to promote colony formation and the colony-forming abilities of T cells varied widely between different normal individuals. Studies in patients with haemopoietic deficiencies showed that the growth of TL-CFC can be used to distinguish between a real lack of colony-forming cells and an apparent reduction, secondary to mononuclear cell depletion.

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.