PMID: 3753620Jan 1, 1986Paper

The use of alkaline elution procedures to measure DNA damage in spermiogenic stages of mice exposed to methyl methanesulfonate

Mutation Research
G A SegaE E Generoso

Abstract

The ability of methyl methanesulfonate (MMS) to induce DNA breakage in spermiogenic stages of the mouse was studied using an alkaline elution technique. At daily intervals over a 3-week period following i.p. injection of 50 mg MMS/kg, mature spermatozoa were recovered from treated (3H-labeled) and control (14C-labeled) animals, lysed together on polycarbonate filters, and eluted with a high pH (12.2) buffer. Elution of germ-cell DNA from MMS-treated animals was found to increase in stages in which genetic damage from MMS is greatest. In general, the pattern of DNA elution from treated, spermiogenic stages paralleled the pattern of sensitivity to dominant lethals, specific-locus mutations and heritable translocations found by other investigators. It also paralleled the pattern of sperm-head methylation and protamine methylation measured in an earlier study (Sega and Owens, 1983). At 9 days post treatment (sperm sampled were in mid-to late-spermatid stages at the time of MMS exposure) the elution of sperm DNA did not change significantly over a pH range of 11.6-12.8, suggesting that, at the time of assay, DNA breaks were already present in the sperm. Because of the parallelism found between increased sperm DNA elution and increas...Continue Reading

References

Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A J FornaceH E Kann
Oct 19, 1976·Biochemistry·K W KohnC A Friedman
Aug 23, 1978·Biochimica Et Biophysica Acta·M O BradleyK W Kohn
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Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L C EricksonK W Kohn
Nov 1, 1980·Archives of Toxicology·U H Ehling

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Citations

Dec 12, 2003·Fertility and Sterility·Narendra P SinghRichard E Berger
Jun 4, 1999·Mutation Research·M S MartyB B Gollapudi
Jan 1, 1993·Environmental and Molecular Mutagenesis·D P EvensonR K Baer

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