The use of amphipols as universal molecular adapters to immobilize membrane proteins onto solid supports.

Proceedings of the National Academy of Sciences of the United States of America
D CharvolinJean-Luc Popot

Abstract

Because of the importance of their physiological functions, cell membranes represent critical targets in biological research. Membrane proteins, which make up approximately 1/3 of the proteome, interact with a wide range of small ligands and macromolecular partners as well as with foreign molecules such as synthetic drugs, antibodies, toxins, or surface recognition proteins of pathogenic organisms. Whether it is for the sake of basic biomedical or pharmacological research, it is of great interest to develop tools facilitating the study of these interactions. Surface-based in vitro assays are appealing because they require minimum quantities of reagents, and they are suitable for multiplexing and high-throughput screening. We introduce here a general method for immobilizing functional, unmodified integral membrane proteins onto solid supports, thanks to amphipathic polymers called "amphipols." The key point of this approach is that functionalized amphipols can be used as universal adapters to associate any membrane protein to virtually any kind of support while stabilizing its native state. The generality and versatility of this strategy is demonstrated by using 5 different target proteins, 2 types of supports (chips and beads),...Continue Reading

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Citations

May 7, 2011·Annual Review of Biophysics·J-L PopotM Zoonens
Apr 19, 2011·Trends in Biotechnology·Jean-Louis BanèresBernard Mouillac
Oct 23, 2015·Biomacromolecules·Fabrice GiustiManuela Zoonens
Aug 5, 2014·The Journal of Membrane Biology·Yann FerrandezPhilippe Minard
Mar 22, 2014·The Journal of Membrane Biology·Fabrice GiustiJean-Luc Popot
Apr 4, 2014·The Journal of Membrane Biology·Christel Le BonFabrice Giusti
Apr 15, 2014·The Journal of Membrane Biology·Eduardo Antonio Della PiaKaren L Martinez
Sep 7, 2014·The Journal of Membrane Biology·V PolovinkinV Gordeliy
May 20, 2014·The Journal of Membrane Biology·Zhibin NingDaniel Figeys
Jun 6, 2014·The Journal of Membrane Biology·A FernandezD Bagnard
Jun 28, 2014·The Journal of Membrane Biology·Manuela Zoonens, Jean-Luc Popot
Jun 12, 2013·Protein Science : a Publication of the Protein Society·Wei HeMatthew A Coleman
Nov 1, 2011·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jan 31, 2014·ACS Nano·Eduardo Antonio Della PiaKaren Laurence Martinez
Apr 17, 2013·Biotechnology and Applied Biochemistry·Tomas LaursenBirger Lindberg Møller
Jun 4, 2011·Biopolymers·Tassadite DahmaneJean-Luc Popot
May 5, 2018·Frontiers in Molecular Biosciences·Montserrat Serra-BatisteNatàlia Carulla
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Aug 27, 2011·Journal of the American Chemical Society·Anders GunnarssonFredrik Höök
Feb 12, 2013·Journal of Proteome Research·Zhibin NingDaniel Figeys

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