PMID: 9446824Apr 4, 1998Paper

The use of avidin as a probe for the distribution of mitochondrial carboxylases in developing chick retina

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
F P Ruggiero, J B Sheffield

Abstract

During development, the inner chick retina progresses from an aerobic to an anaerobic metabolic basis because of the lack of a vascular system. To investigate this process further, we have examined the expression and distribution of mitochondrial carboxylases. Because these enzymes use covalently bound biotin as a co-enzyme, we were able to develop a new detection protocol for mitochondria using avidin as a probe for the biotin. Chemiluminescent detection of bound avidin-peroxidase was used to examine a developmental series of extracts of retinas that had been separated by electrophoresis and blotted to nitrocellulose. Avidin-peroxidase, visualized with the sensitive peroxidase substrate True Blue, permitted detection in epoxy-embedded tissue sections. In the extracts, specific bands of approximate molecular weights 130 and 70 kD were found, corresponding to biotinylated subunits of several mitochondrial carboxylases. During development, the intensity of the bands decreases, although at different rates. In tissue sections, 8-day embryonic retinas display reaction product throughout the tissue, with higher local concentrations in the vitread and sclerad regions. During further development, the reaction product becomes segregated...Continue Reading

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Citations

Mar 23, 2000·Brain Research. Developmental Brain Research·F JohnsonK Soderstrom
Jun 6, 2000·Brain Research. Developmental Brain Research·Y Ban, L J Rizzolo
Sep 11, 2003·Journal of Neuroimmunology·Nison SattayasaiHathairat Puchongkavarin
Dec 31, 2004·Organic Letters·Tae Woo KimJong-In Hong
Oct 25, 2011·PloS One·Tobias KohlWalter Stühmer
Jun 9, 2004·Experimental Eye Research·Kathleen M LeahyMartin B Wax

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