PMID: 6539298Jan 1, 1984Paper

The use of cloned Y chromosome-specific DNA probes for fetal sex determination in first trimester prenatal diagnosis

Human Genetics
J R GosdenC H Rodeck

Abstract

Prenatal diagnosis by chorion biopsy in the first trimester of pregnancy has advantages over second trimester amniocentesis because diagnosis can be achieved at 9-12 weeks gestation, reducing prenatal anxiety and avoiding the trauma of late abortion. DNA can be prepared from chorionic villus biopsies in sufficient quantity and purity for use in prenatal diagnosis systems using specific DNA probes hybridised to restriction endonuclease digests. DNA probes derived from the Y chromosome have been used to determine fetal sex. The use of such probes means that the chromosomal sex of the fetus can be identified more quickly than by chromosome preparation and more accurately than by sex chromatin staining, and has the additional advantage that the same DNA preparation can be used for other diagnostic tests. A dot hybridisation method has been successfully used to provide even more rapid results than conventional hybridisation to Southern blots of restriction endonuclease digests. There is a risk that Y chromosome-specific DNA probes for sex determination may be subject to error if the parents have extreme Y chromosome variants such as a small or non-fluorescent Y or a Y autosome chromosome translocation. The precise extent to which su...Continue Reading

References

Apr 1, 1975·Experimental Cell Research·J R GosdenH J Evans
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·Y W Kan, A M Dozy
Jan 1, 1979·Human Genetics·H J Cooke, B Noel
Jan 1, 1974·Journal of Clinical Pathology. Supplement (Royal College of Pathologists)·D J Brock
Oct 1, 1983·British Medical Bulletin·C H Rodeck, J M Morsman
Oct 1, 1983·British Medical Bulletin·C M Gosden
Dec 1, 1983·British Journal of Obstetrics and Gynaecology·C H RodeckJ R Gosden

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Citations

Jul 1, 1986·Indian Journal of Pediatrics·I Bartels, I Hansmann
Jun 26, 1989·Nucleic Acids Research·R C AllshireN D Hastie
Feb 1, 1987·Journal of Medical Genetics·A C ChandleyR M Speed
Jan 1, 1985·Journal of Inherited Metabolic Disease·H J SipsE E Sterchi
Feb 1, 1989·Acta Paediatrica Japonica; Overseas Edition·T YorifujiT Mori
Jan 1, 1989·Molecular Reproduction and Development·J D WestR J Aitken
Jun 1, 2006·FEMS Microbiology Letters·Sylvie MazurierPhilippe Lemanceau
Dec 1, 1989·Jinrui idengaku zasshi. The Japanese journal of human genetics·K NagaiI Yanagisawa

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