The use of coenzyme Q0 as a template in the development of a molecularly imprinted polymer for the selective recognition of coenzyme Q10

Analytica Chimica Acta
Mario ContinValeria Tripodi

Abstract

In this work, a novel molecularly imprinted polymer (MIP) for use as a solid phase extraction sorbent was developed for the determination of coenzyme Q10 (CoQ10) in liver extract. CoQ10 is an essential cofactor in mitochondrial oxidative phosphorylation and a powerful antioxidant agent found in low concentrations in biological samples. This fact and its high hydrophobicity make the analysis of CoQ10 technically challenging. Accordingly, a MIP was synthesised using coenzyme Q0 as the template, methacrylic acid as the functional monomer, acetonitrile as the porogen, ethylene glycol dimethacrylate as the crosslinker and benzoyl peroxide as the initiator. Various parameters affecting the polymer preparation and extraction efficiency were evaluated. Morphological characterisation of the MIP and its proper comparison with C18 as a sorbent in solid phase extraction were performed. The optimal conditions for the molecularly imprinted solid phase extraction (MISPE) consisted of 400 μL of sample mixed with 30 mg of MIP and 600 μL of water to reach the optimum solution loading. The loading was followed by a washing step consisting of 1 mL of a 1-propanol solution (1-propanol:water, 30:70,v/v) and elution with 1 mL of 1-propanol. After cle...Continue Reading

References

Apr 11, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·He DongLong-di Li
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May 6, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Afshin Rajabi Khorrami, Shima Mehrseresht
Sep 17, 2010·Analytical and Bioanalytical Chemistry·Bernadette Tse Sum Bui, Karsten Haupt
Oct 4, 2011·Journal of Pharmacy & Bioallied Sciences·Rajiv Saini

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Citations

Feb 17, 2015·Future Medicinal Chemistry·Mariacristina Gagliardi, Barbara Mazzolai
Dec 19, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wenhua JiLuqi Huang
Oct 6, 2018·Journal of Separation Science·Martina HavlikovaZuzana Bosakova
May 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kangde BaoChengxi Jiang

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