PMID: 7523474Oct 1, 1994Paper

The use of cutaneous microdialysis to measure substance P-induced histamine release in intact human skin in vivo

The Journal of Allergy and Clinical Immunology
L J PetersenP S Skov

Abstract

The purpose of this study was to introduce a microdialysis technique, which makes it possible to measure the release of small inflammatory mediators into the extracellular water space in intact human skin in vivo. Using this technique, we have studied the histamine releasing properties of substance P, a putative skin mast cell releasing agent. Small hollow fibers were inserted into the upper dermis of nine healthy subjects. Each fiber was perfused with Kreb's Ringer bicarbonate buffer at a rate of 3.0 microliters/min. After establishment of a baseline, each fiber was challenged intracutaneously with substance P (0 to 4 mumol/L). Samples were collected at 2-minute intervals for 18 minutes. Histamine was measured by a fluorometric method, which correlated with an enzyme immunoassay (r = 0.96). Baseline dialysate histamine concentration was 1.7 +/- 0.3 ng/ml. Peak histamine release after injection of vehicle, 0.5, 1, 2, and 4 mumol/L substance P was 0.0, 1.0, 6.0, 44.5, and 88.5 ng/ml, respectively (p = 0.00002). Statistically significant histamine release was demonstrated with 1.0 mumol/L substance P and greater. Most peak values were seen 2 to 4 minutes after injection. The histamine elimination showed a monoexponential decline;...Continue Reading

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Citations

Sep 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·M PerzanowskaM K Church
Mar 7, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Lars J PetersenHenrik Kehlet
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Jul 27, 1999·The British Journal of Dermatology·A L KrogstadB G Wallin
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Mar 26, 2003·European Journal of Anaesthesiology·J A BlunkW Koppert
Aug 1, 1997·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L J PetersenP S Skov
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Apr 5, 2013·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Lars J PetersenEmøke Bendixen
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Mar 1, 1997·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L J PetersenP S Skov
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