PMID: 735304Oct 31, 1978

The use of deep frozen, stored bovine blood for in vitro feeding of tsetse flies

Zeitschrift Für Parasitenkunde
H Wetzel, D Luger

Abstract

A method of blood preparation is described which resulted in the successful rearing of Glossina p. palpalis with in vitro feeding. Cells of defibrinated bovine blood were washed in 0.89% NaCl solution prior to deep-freezing. Cells and serum were stored (--28 degrees C) for up to four months. For feeding, both components were mixed in the volumetric proportion 1 : 1. Adenosine triphosphate was added at a concentration of 10-3 M to stimulate uptake of blood. Survival rate and mean weight of puparia remained constant over three generations while productivity increased.

References

Aug 1, 1976·The Australian Journal of Experimental Biology and Medical Science·B V Goodger
Jan 1, 1973·Transactions of the Royal Society of Tropical Medicine and Hygiene·A R MewsE D Offori
Sep 22, 1971·Nature: New Biology·R Galun, M J Rice

Related Concepts

Striadyne
Metazoa
Animal Feed (Substance)
Blood
Breeding
Bos indicus
Melting
Pupa
Glossina

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