PMID: 735304Oct 31, 1978

The use of deep frozen, stored bovine blood for in vitro feeding of tsetse flies

Zeitschrift Für Parasitenkunde
H Wetzel, D Luger


A method of blood preparation is described which resulted in the successful rearing of Glossina p. palpalis with in vitro feeding. Cells of defibrinated bovine blood were washed in 0.89% NaCl solution prior to deep-freezing. Cells and serum were stored (--28 degrees C) for up to four months. For feeding, both components were mixed in the volumetric proportion 1 : 1. Adenosine triphosphate was added at a concentration of 10-3 M to stimulate uptake of blood. Survival rate and mean weight of puparia remained constant over three generations while productivity increased.


Aug 1, 1976·The Australian Journal of Experimental Biology and Medical Science·B V Goodger
Jan 1, 1973·Transactions of the Royal Society of Tropical Medicine and Hygiene·A R MewsE D Offori
Sep 22, 1971·Nature: New Biology·R Galun, M J Rice

Related Concepts

Animal Feed (Substance)
Bos indicus

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

© 2021 Meta ULC. All rights reserved