The use of detergents to purify membrane proteins

Current Protocols in Protein Science
Thomas Arnold, Dirk Linke

Abstract

Extraction of membrane proteins from biological membranes is usually accomplished with the help of detergents. This unit describes the use of detergents to solubilize and purify membrane proteins. The chemical and physical properties of the different classes of detergents typically used with biological samples are discussed. A separate section addresses the compatibility of detergents with applications downstream of the membrane protein purification process, such as optical spectroscopy, mass spectrometry, protein crystallography, or biomolecular NMR. Protocols in this unit include the isolation and solubilization of biological membranes, phase separation, and support protocols for detergent removal and detergent exchange using different methods.

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Citations

Sep 25, 2016·Food Chemistry·Angelica Tamayo TenorioAtze Jan van der Goot
Oct 21, 2011·Journal of Mass Spectrometry : JMS·Mridul Kanti MandalKenzo Hiraoka
Oct 26, 2012·Biopolymers·Arjun ThapaGabriel P López
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Sep 13, 2014·Environmental Microbiology·Karin AistleitnerMatthias Horn
Sep 25, 2016·Biological Chemistry·Susann ZilkenatSamuel Wagner
Dec 31, 2020·International Journal of Molecular Sciences·Johannes Thoma, Björn M Burmann
Sep 30, 2017·The Journal of Physical Chemistry Letters·Ryan C OliverVolker S Urban
Nov 7, 2021·Environmental Science and Pollution Research International·Shuai KongQin Meng

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