PMID: 8950600Oct 1, 1996Paper

The use of enhanced polymer one-step staining reagents for immunoenzyme double-labelling

The Histochemical Journal
C M Van der LoosA E Becker

Abstract

The newly developed peroxidase-labelled Enhanced Polymer One-Step (EPOS) reagents were applied, together with an unlabelled primary mouse antibody, in a multistep double-labelling protocol. Enzyme label reporter combinations consisted of either peroxidase and alkaline phosphatase in red and blue, respectively, or beta-galactosidase and alkaline phosphatase in turquoise and red, respectively. The latter enzyme combination was introduced using a rabbit antiperoxidase antibody and an enzyme-labelled anti-rabbit immunoglobulin antibody. The multistep procedure was tested using five different antibody combinations on cryostat and Carnoy- or formalin-fixed, paraffin-embedded sections. In each instance, clear and distinct labelling was obtained, either with the two antigens at separate sites, or with an overlap in distribution. In the latter situation, the sites of co-localization were marked by mixed colours, which were distinct and readily discriminated from the two basic colours.

References

Nov 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger, S A Joseph
Nov 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C M van der LoosH J Houthoff
Aug 1, 1988·The Histochemical Journal·C M van der LoosH J Houthoff
Sep 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane
Jan 1, 1982·Histochemistry·A BondiG Bussolati
Oct 1, 1993·The Journal of Pathology·G CattorettiE Feudale
Jan 1, 1993·The Histochemical Journal·C M van der LoosJ J van den Oord
Feb 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C GRAHAMM J KARNOVSKY

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Citations

Nov 27, 2014·Archives of Pathology & Laboratory Medicine·Fan Lin, Zongming Chen
Feb 24, 2001·Progress in Histochemistry and Cytochemistry·G Mayer, M Bendayan
Sep 25, 2002·The European Journal of Neuroscience·Carlo ZancanaroAndrea Cavaggioni
Dec 19, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H A LehrA M Gown
Feb 12, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K A RothR G Lorenz
Jul 26, 2019·Cellular and Molecular Life Sciences : CMLS·Karolina BoguszewskaBolesław T Karwowski
Oct 31, 2008·Current Protocols in Molecular Biology·James R MansfieldRichard M Levenson

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