PMID: 7008641Sep 1, 1980Paper

The use of enzyme-linked immunosorbent assay (ELISA) in the diagnosis of farmer's lung

Allergy
T H OjanenR Mäntyjärvi

Abstract

Enzyme-linked immunosorbent assay (ELISA) was adapted to measure antibodies against Aspergillus fumigatus, Thermoactinomyces vulgaris and Micropolyspora faeni. Mycelial and culture fluid antigens were used separately to analyze antibodies in three study groups: farmer's lung patients, patients with bronchitis and healthy control persons. The mycelial antigen of A. fumigatus gave more positive results than the corresponding culture fluid antigen. The situation was reversed for the actinomycetes. For this reason either a mycelial antigen or a combination of mycelial and culture fluid antigens should be used in ELISA in routine tests. The prevalence and the titers of antibodies against the three microbes detected by ELISA were higher in the farmer's lung group than in the other two groups.

References

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Citations

Jan 19, 1999·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·S LappalainenP Kalliokoski
Apr 1, 1985·Sabouraudia·H SchønheyderA Stenderup
Oct 1, 1993·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K KaukonenE O Terho
Feb 1, 1984·British Journal of Industrial Medicine·M S HuuskonenR Mäntyjärvi
May 1, 1992·British Journal of Industrial Medicine·T Ojanen
Jan 1, 1982·Critical Reviews in Toxicology·R P StankusJ E Salvaggio
Jan 1, 1987·Scandinavian Journal of Infectious Diseases. Supplementum·H Schønheyder
Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·P S CraigT Romig
Jan 1, 1984·Transactions of the Royal Society of Tropical Medicine and Hygiene·R J Dahl, A M Johnson
Mar 1, 1984·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section A, Pathology·A SeppäR A Mäntyjärvi

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