The use of equine chondrogenic-induced mesenchymal stem cells as a treatment for osteoarthritis: A randomised, double-blinded, placebo-controlled proof-of-concept study

Equine Veterinary Journal
Sarah BroeckxJan H Spaas

Abstract

There is a need to improve therapies for osteoarthritis in horses. To assess the efficacy of equine allogeneic chondrogenic-induced mesenchymal stem cells combined with equine allogeneic plasma as a novel therapy for osteoarthritis in horses. Randomised, double-blinded, placebo-controlled experiment. In 12 healthy horses, osteoarthritis was induced in the metacarpophalangeal joint using an osteochondral fragment-groove model. Five weeks after surgery, horses were randomly assigned to either an intra-articular injection with chondrogenic-induced mesenchymal stem cells + equine allogeneic plasma (= intervention) or with 0.9% saline solution (= control). From surgery until the study end, horses underwent a weekly joint and lameness assessment. Synovial fluid was collected for cytology and biomarker analysis before surgery and at Weeks 5, 5 + 1d, 7, 9 and 11. At Week 11, horses were subjected to euthanasia, and the metacarpophalangeal joints were evaluated macroscopically and histologically. No serious adverse events or suspected adverse drug reactions occurred during the study. A significant improvement in visual and objective lameness was seen with the intervention compared with the control. Synovial fluid displayed a significant...Continue Reading

References

Jan 24, 2006·The Veterinary Journal·Laurie R Goodrich, Alan J Nixon
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Sep 2, 2014·Current Stem Cell Research & Therapy·Sarah BroeckxJan H Spaas
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Jan 16, 2016·Veterinary Surgery : VS·Henry W JannBrenda K Mann
Feb 26, 2019·American Journal of Veterinary Research·Sarah Y BroeckxAnn M Martens

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Citations

Mar 22, 2020·Animals : an Open Access Journal From MDPI·Anne Franck Gallagher Vom BrauckeCasper Lindegaard
Sep 27, 2020·Animals : an Open Access Journal From MDPI·Ki Hoon KimTae Min Kim
Sep 10, 2020·Frontiers in Bioengineering and Biotechnology·Iris RibitschLouis C Penning
Jan 23, 2021·Animals : an Open Access Journal From MDPI·Iris RibitschFlorien Jenner
Dec 16, 2020·Animals : an Open Access Journal From MDPI·Aleksandra ZygmuntowiczWłodzimierz Markiewicz
Jun 23, 2021·Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere·Susanne P RothAntonia Troillet

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Methods Mentioned

BETA
sedation
ELISA

Software Mentioned

SAS
Lameness Locator
LAS
Equinosis Q
The

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