PMID: 20650091Jun 1, 1995Paper

The use of freshly isolated gill epithelial cells in toxicity testing

Toxicology in Vitro : an International Journal Published in Association with BIBRA
H LiliusB Isomaa

Abstract

The large number of chemicals in our environment, many of which have been insufficiently tested for toxicity, justifies the need for rapid and reliable in vitro testing to predict toxicity. In aquatic toxicology the use of cellular toxicity tests has been limited. In the present study, the usefulness of freshly isolated gill epithelial cells from rainbow trout in toxicity testing was evaluated by testing the cytotoxicity of 30 reference chemicals to the cells using the fluorescent cell viability probe Calcein-AM. The results were compared with previously obtained EC(50) values for the chemicals in freshly isolated rainbow trout hepatocytes, EC(50) values for immobilization of Daphnia magna and literature data on LC(50) values for fish. Regression analysis of the data obtained with gill epithelial cells and hepatocytes showed a correlation coefficient of 0.77 (r(2) = 0.59). The gill epithelial cells were slightly more sensitive than hepatocytes to the chemicals. The slope of the regression line was 0.79. Both cell types were less sensitive than D. magna and fish to the chemicals, but the EC(50) values for gill epithelial cells showed a better concordance with EC(50) values for immobilization in D. magna and with literature data ...Continue Reading

References

Jan 1, 1983·Annals of the New York Academy of Sciences·B Ekwall

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Citations

Apr 29, 2011·Archives of Environmental Contamination and Toxicology·Caroline T Rocha, Marta M Souza
Feb 3, 2009·In Vitro Cellular & Developmental Biology. Animal·L E J LeeN C Bols
Jun 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H LiliusB Isomaa
Mar 4, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M SandbackaB Isomaa
Mar 23, 2011·Environmental Toxicology and Chemistry·Thaís Martins LopesAdalto Bianchini
Nov 29, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Xiao-yong ChenXin-mei Liu
Mar 23, 2011·Environmental Toxicology and Chemistry·Thaís Martins LopesAdalto Bianchini
Oct 10, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Martina GiannacciniVittoria Raffa
Dec 23, 2004·Alternatives to Laboratory Animals : ATLA·Argelia CastañoUNKNOWN ECVAM Workshop 47
May 1, 2000·Alternatives to Laboratory Animals : ATLA·M SandbackaB Isomaa
Jan 18, 2005·Alternatives to Laboratory Animals : ATLA·Helmut Segner
Feb 28, 2014·The Journal of Experimental Biology·Nic R BuryChrister Hogstrand

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