The use of FTA cards for transport and detection of gyrA mutation of Campylobacter jejuni from poultry

Poultry Science
Yuli Melisa Sierra-ArguelloVladimir Pinheiro do Nascimento

Abstract

The purpose of the present study was to evaluate a technique involving the use of commercially available FTA classic card (Whatman) for transporting and detection of DNA to use in PCR analysis and genetic sequencing of Campylobacter jejuni of poultry origin. Fifty isolates of Campylobacter jejuni were obtained from broiler carcasses in Rio Grande do Sul, Brazil. Antimicrobial susceptibility testing to ciprofloxacin revealed that all 50 isolates were resistant to ciprofloxacin. Each isolate was transferred to Brucella broth tubes and incubated overnight at 41.5°C. Cell cultures were diluted to match a McFarland Turbidity Standard 0.5, and 110 μL of the cell suspension were applied to one circle on Whatman FTA classic cards. The samples were then covered and allowed to dry at room temperature. Cards were identified and stored at room temperature until further use (3 mo after collection). FTA cards were shipped for analysis to the Department of Poultry Science, University of Arkansas. Amplification of the Campylobacter gyrA gene was successful and demonstrated strong bands for a large amplicon for all 50 samples preserved on FTA cards. Mutations present in each gene were confirmed by DNA sequencing. Then, 7 samples were chosen for...Continue Reading

References

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Citations

Sep 16, 2016·Frontiers in Veterinary Science·Eduardo Morales-BarreraGuillermo Tellez
Sep 27, 2018·Journal of Clinical Gastroenterology·Di ZhangMarina Elli
Oct 15, 2019·Preventive Veterinary Medicine·Jaime A Cardona-OspinaJuan C Sepúlveda-Arias

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Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

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