The use of glutaraldehyde-fixed chicken red blood cells as counting beads for performing affordable single-platform CD4(+) T-lymphocyte count in HIV-1-infected patients

Journal of Acquired Immune Deficiency Syndromes : JAIDS
Kovit PattanapanyasatBoonrat Tassaneetrithep

Abstract

CD4(+) T-lymphocyte count is an important marker in management of HIV-1-infected patients. The standard single-platform (SP) flow cytometric (FCM) CD4(+) testing that uses the known reference microbeads is expensive; more affordable alternatives are therefore needed. We evaluated the use of glutaraldehyde-fixed chicken red blood cells (CRBCs) as counting beads as an alternative for enumerating CD4(+) T-lymphocyte counts in 87 HIV-1-infected patients. Linear regression analyses revealed an excellent correlation of the SP FCM using CRBCs with the standard SP bead-based FCM method (percentages, r(2) > 0.99; absolute counts, r(2) > 0.98) over the entire range including the clinically relevant range. Mean percent bias for the CRBC method was +0.35% [limits of agreement (LOA): -1.86% to +2.57%]. For absolute CD4(+) T-lymphocytes, the mean biases was -47.76 cells per microliter (LOA: -191.34 to +98.81 cells/microL) with much lower bias for CD4 T-lymphocyte counts <200 cells per microliter (LOA: -31.92 to +22.95 cells/microL). The use of CRBCs is comparable with the use of commercial microbeads. This has resulted in major cost savings to resource-limited countries where the health care system is under increasing pressure to operate cos...Continue Reading

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Feb 3, 2009·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Kovit PattanapanyasatBoonrat Tassaneetrithep

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Citations

Mar 26, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Yang YuDeqing Wang

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