PMID: 49342Jul 30, 1975Paper

The use of glycol methacrylate embedding for studies of chromatin distribution and fine structure in salmon spermatid nuclei

Histochemistry
B R Zirkin

Abstract

Glycol methacrylate has been used as an embedding medium for studies of spermiogenesis in the salmon. DNA and basic proteins are shown to stain with the same specificity in thick (0.5-1.0 mu) sections of GMA-embedded salmon testes as in sections of comparably fixed, paraffin-embedded testes. Stain can be localized far more precisely in GMA sections than in paraffin sections due to the thinness of the sections and to the excellent structural preservation of nuclei. In addition, ultra-thin sections of GMA-embedded salmon testes can be observed with the electron microscope, and this permits exact correlation between nuclear fine structure and chemical composition in consecutive sections of the same nuclei.

References

Feb 1, 1975·Journal of Ultrastructure Research·B R Zirkin
Sep 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Hoshino, H Kobayashi
Dec 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A E AshfordM E McCully
Feb 1, 1974·Journal of Ultrastructure Research·B Lundgren, M Westin
Jul 1, 1967·Journal of Ultrastructure Research·E H Leduc, W Bernhard
Jan 1, 1971·Journal of Ultrastructure Research·B R Zirkin
Mar 25, 1956·The Journal of Biophysical and Biochemical Cytology·M Alfert
Jun 1, 1961·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·H RIS
Dec 1, 1960·Journal of Ultrastructure Research·M ROSENBERGJ LESKO
Jun 1, 1960·The Journal of Biophysical and Biochemical Cytology·D P BLOCH, H Y HEW
Oct 1, 1953·Proceedings of the National Academy of Sciences of the United States of America·M Alfert, I I Geschwind

Citations

Jan 1, 1985·Progress in Histochemistry and Cytochemistry·J A Litwin
Jul 1, 1981·Journal of Microscopy·M B Cole, J Ellinger

Related Concepts

Cell Nucleus
Chromatin
DNA, Double-Stranded
Glycols
Histological Techniques
Histone H7
Methacrylates
Salmo salmo
Spermatid
Spermiogenesis

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