The use of gold chloride to stain developing and adult neuromuscular junctions in the insect.

Stain Technology
M B Rheuben, P J Schaner

Abstract

Individual insect muscle fibers, whose neuromuscular junctions have been stained with a modification of Ranvier's gold chloride method, can be dissected free and mounted whole if the muscle is prefixed in aldehydes. The neuromuscular junctions along the length of the individual fibers are well delineated and can be measured and counted. Effective procedures include fixation with glutaraldehyde buffered to low pH with sodium citrate, or glutaraldehyde and paraformaldehyde combined in phosphate buffer at neutral pH, followed by exposure to citric acid and to gold chloride. The method is convenient, and could be useful for the study of arthropod neuromuscular junctions in general, since their nerve terminals do not release acetylcholine as a transmitter and cannot be stained by the more commonly used cholinesterase methods.

References

Nov 1, 1978·Journal of Ultrastructure Research·M B Rheuben, T S Reese
Apr 27, 1971·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·U J McMahan, S W Kuffler
Oct 1, 1972·The Journal of Cell Biology·J L Oschman, B J Wall
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L Y Jan, Y N Jan
Jan 1, 1946·Stain Technology·W V COLE

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